Cloning and characterization of polyA- RNA transcripts encoded by activated B1-like retrotransposons in mouse erythroleukemia MEL cells exposed to methylation inhibitors

BMB Reports
Sotirios S TeziasI S Vizirianakis

Abstract

We have previously identified a DNA silent region located downstream of the 3'-end of the β(major) globin gene (designated B1-559) that contains a B1 retrotransposon, consensus binding sites for erythroid specific transcription factors and shares the capacity to act as promoter in hematopoietic cells interacting with β-globin gene LCR sequences in vitro. In this study, we have cloned four new non-polyA RNA transcripts being detected upon blockade of murine erythroleukemia (MEL) cell differentiation to erythroid maturation by methylation inhibitors and demonstrated that two of them share high structural homology with sequences of B1 element found within the B1-559 region. Although it is not clear yet whether and how these RNAs interfere with induction of erythroid maturation, these data provide evidence for the first time showing that methylation inhibitors can activate silent repetitive DNA sequences in MEL cells and may have implications in cancer chemotherapy using demethylating drugs as antineoplastic agents.

References

May 3, 2003·Oncology Research·Asterios S TsiftsoglouIoannis S Vizirianakis
Dec 4, 2003·Pharmacology & Therapeutics·Asterios S TsiftsoglouIoannis S Vizirianakis
May 14, 2009·International Journal of Cancer. Journal International Du Cancer·Si Ho ChoiAllen S Yang
Jul 22, 2009·IUBMB Life·Asterios S TsiftsoglouJohn Strouboulis
Nov 19, 2010·Nature·Alysson R MuotriFred H Gage
Dec 15, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Cora Mund, Frank Lyko

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