Cloning and characterization of polyphenol oxidase cDNAs of Phytolacca americana

Plant Physiology
R W JoyA Komamine

Abstract

Two cDNA clones encoding polyphenol oxidases were isolated from a cDNA library constructed from a log-phase suspension culture of Phytolacca americana (pokeweed) producing betalains. The clones exhibit 93 and 86% sequence identity at the nucleotide and deduced amino acid levels, respectively. Both clones contain two copper-binding domains characterized by histidine-rich regions, which are found ubiquitously in all polyphenol oxidases/tyrosinases, and a putative third histidine-rich, copper-binding region, which is common to all plant polyphenol oxidases. One of the Phytolacca cDNA deduced amino acid sequences contains the ubiquitous transit peptide for all proteins targeted to the internal lumen of thylakoid membranes of plastids and is considered to be 98 residues in length based on a proposed sequence cleavage site motif. This would produce a processed peptide of approximately 54 kD. In addition to common features of transit peptides, it was found that an additional conserved region for polyphenol oxidases was located between the hydroxy amino acid-rich region and the thylakoid transfer domain. Spatial and temporal expression was investigated by northern blot analysis of total RNA from various organs of Phytolacca plants. Tra...Continue Reading

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Citations

Oct 23, 2001·Angewandte Chemie·Dieter Strack, Willibald Schliemann
Mar 7, 2003·Phytochemistry·Dieter StrackWillibald Schliemann
Jan 1, 2003·Journal of Experimental Botany·Sylviane ComparotThomas Martin
Apr 10, 1999·Plant Physiology·T ChevalierB R Fils-Lycaon
May 23, 2012·Evidence-based Complementary and Alternative Medicine : ECAM·Manyatja Brenda MapunyaNamrita Lall
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Feb 12, 2013·Trends in Plant Science·Fernando Gandía-Herrero, Francisco García-Carmona
Jun 24, 2015·Phytochemistry·Mohammad Imtiyaj Khan, P Giridhar
Jun 14, 2013·Scientific Reports·Takashi NakatsukaMasahiro Nishihara
May 15, 2008·The Plant Journal : for Cell and Molecular Biology·Yoshikazu TanakaAkemi Ohmiya
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Eiichiro OnoToru Nakayama
Jun 30, 2011·The New Phytologist·Samuel F BrockingtonDouglas E Soltis
Oct 1, 2016·International Journal of Molecular Sciences·Qingzhu HuaYonghua Qin
Jan 1, 2015·3 Biotech·Aravind Kumar Jukanti, Ramakrishna Bhatt
Aug 1, 1997·Phytochemistry·C W van GelderH J Wichers

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