Cloning and characterization of rainbow trout (Oncorhynchus mykiss) somatolactin cDNA and its expression in pituitary and nonpituitary tissues

General and Comparative Endocrinology
B Y YangT T Chen

Abstract

A cDNA clone encoding rainbow trout (Oncorhynchus mykiss) somatolactin (rtSL) has been isolated from a rainbow trout pituitary cDNA library. This 2329-bp cDNA clone includes a very short 7-bp 5'-untranslated region, a coding region of 702 bp, and a long 3'-untranslated region of 1620 bp. The deduced amino acid sequence of rtSL shows a polypeptide of 233 amino acid residues which consists of a 24-amino-acid putative signal peptide followed by a 209-amino-acid mature polypeptide. This mature polypeptide has a molecular weight of 24 kDa. The rtSL shares 99% amino acid identity with chum salmon SL (csSL) and approximately 53-77% amino acid identity with SLs in other fishes, including the 7 conserved cysteine residues. Although a glycosylation site has been identified in SL of other fish species, none is observed in rtSL polypeptide. The level of rtSL mRNA in a single pituitary gland was determined by RNA blot hybridization. Results showed that levels of SL mRNA in pituitary glands of 2-year-old fish were 4- to 7-fold higher than those of 1-year-old fish. The tissue distribution of SL gene expression in adult fish was determined by reverse transcription-polymerase chain reaction (RT-PCR) and DNA blot hybridization. In addition to th...Continue Reading

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