Cloning and characterization of rat spermatid protein SSP411: a thioredoxin-like protein

Journal of Andrology
Hui-Juan ShiChing-Ling C Chen

Abstract

In an attempt to identify new sperm-specific genes that are involved in sperm maturation, fertilization, and embryo development, such as the mammalian ortholog of the sperm-supplied protein gene, spe-11, in Caenorhabditis elegans, we cloned and characterized a new spermatid-specific protein gene, ssp411, from adult rat testes. The ssp411 cDNA shared >85% sequence identity with an unnamed human protein, FLJ21347, and an uncharacterized mouse testicular protein called transcript increased in spermiogenesis 78 (TISP78). A 2.8-kb ssp411 mRNA was expressed in a testis-specific and age-dependent manner; the mRNA was evident at 28 days and remained at high levels throughout adulthood. An SSP411 protein of molecular weight 88 000 was detected in testicular extracts by Western blot analysis. Ssp411 mRNA and SSP411 protein, as analyzed by in situ hybridization and immunohistochemistry, were both expressed in a stage-dependent fashion during the cycle of the seminiferous epithelium. The ssp411 mRNA was predominantly localized to round and elongated spermatids, with maximal expression at stages VII-XII. The SSP411 protein was mainly observed in elongated spermatids and reached its highest levels during stages V-VI. A conserved thioredoxin-...Continue Reading

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Citations

Jul 18, 2020·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S JarvisC L Bevan
Apr 4, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Yan XuChing-Ling C Chen
Oct 1, 2014·Journal of Hepato-biliary-pancreatic Sciences·Hiroaki Haga, Tushar Patel
Aug 14, 2013·Molecular & Cellular Proteomics : MCP·Karen M ChapmanF Kent Hamra
May 31, 2019·PloS One·Matt J NevilleFredrik Karpe
Oct 22, 2019·PLoS Computational Biology·Giancarlo CroceMartin Weigt
Jul 11, 2009·Fertility and Sterility·Con MallidisNeil McClure

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