Oct 19, 2000

Cloning and characterization of SmeDEF, a novel multidrug efflux pump from Stenotrophomonas maltophilia

Antimicrobial Agents and Chemotherapy
A Alonso, J L Martinez

Abstract

Stenotrophomonas maltophilia is a nosocomial bacterial pathogen intrinsically resistant to several antibiotics. The mechanisms involved in this intrinsic multiresistance phenotype are poorly understood. A library of chromosomal DNA from a spontaneous multidrug-resistant S. maltophilia D457R mutant (A. Alonso and J. L. Martinez, Antimicrob. Agents Chemother. 41:1140-1142, 1997) was screened for complementation of erythromycin susceptibility on an antibiotic-hypersusceptible Escherichia coli DeltaacrAB strain. Cloning and further analysis revealed that a 6-kbp region constituting a transcriptional unit was capable of complementing the antibiotic-susceptible phenotype of an E. coli DeltaacrAB strain. We identified three open reading frames, smeD, smeE and smeF, which code for members of the membrane fusion protein, resistance nodulation division, and outer membrane factor families, respectively. Drug susceptibility assays indicated that the SmeDEF system cloned in E. coli mediates resistance to a wide range of antibiotics. Ethidium bromide and norfloxacin accumulation experiments in the presence and in the absence of carbonyl cyanide m-chlorophenylhydrazone showed that this system constitutes a drug efflux pump dependent on the me...Continue Reading

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Mentioned in this Paper

Gene Expression Regulation, Bacterial
Bacterial Proteins
Erythromycin
Alkalescens-Dispar Group
Outer Membrane
Antibiotic throat preparations
Pathogenic Organism
Transcription, Genetic
Antifungal Antibiotics, Topical
Nodulation

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