PMID: 2507521Oct 1, 1989Paper

Cloning and characterization of srfB, a regulatory gene involved in surfactin production and competence in Bacillus subtilis

Journal of Bacteriology
M M Nakano, P Zuber

Abstract

A Tn917 insertion mutation srfB impairs the production of the lipopeptide antibiotic surfactin in Bacillus subtilis. srfB is located between aroG and ald in the B. subtilis genome, as determined by phage PBS1 transduction mapping, and is not linked to the previously described surfactin loci sfp or srfA. A srfB mutant was found to be also deficient in the establishment of competence. SP beta phage-mediated complementation analysis showed that both competence and surfactin production were restored in the srfB mutant by a single DNA fragment of 1.5 kilobase pairs. The sequence of the complementing DNA revealed that the srfB gene is comA, an early competence gene which codes for a product similar to that of the activator class of bacterial two-component regulatory systems. The srfB mutation impaired the expression of a srfA-lacZ fusion, suggesting that surfactin production is positively regulated at the transcriptional level by the srfB (comA) gene product.

References

Jun 1, 1977·Bacteriological Reviews·E Katz, A L Demain
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1985·Journal of Bacteriology·M S RosenkrantzA L Sonenshein
Jun 1, 1985·Microbiological Reviews·P J Piggot, J A Hoch
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·K A TrachJ A Hoch
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J B Perkins, P J Youngman
Apr 1, 1986·Journal of Bacteriology·M YangD J Henner
Aug 1, 1986·Journal of Bacteriology·M A Vandeyar, S A Zahler
Sep 1, 1986·Journal of Bacteriology·C Hernández-ChicoF Moreno
Jul 1, 1987·Journal of Bacteriology·D W DingmanA L Sonenshein
Jul 1, 1987·Journal of Bacteriology·M AlbanoD Dubnau
May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·F A FerrariJ A Hoch
Jan 1, 1987·Annual Review of Microbiology·H Kleinkauf, H von Döhren
Jul 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·S Tabor, C C Richardson
Sep 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·P E LoveR E Yasbin
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·P J YoungmanR Losick

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Citations

Jul 27, 1992·FEBS Letters·M A Marahiel
Mar 1, 1993·Molecular Microbiology·M A MarahielP Zuber
Jul 9, 2014·Applied and Environmental Microbiology·Susanne MüllerJohn R Kirby
Jan 1, 1991·Molecular Microbiology·D Dubnau
Nov 10, 2009·The FEBS Journal·Timothy L FoleyMichael D Burkart
Feb 23, 2011·International Journal of Molecular Sciences·Niran RoongsawangMasaaki Morikawa
Jan 1, 1990·Critical Reviews in Biotechnology·M M Nakano, P Zuber
Sep 7, 2017·Annual Review of Genetics·Matthew B NeiditchMichael J Federle
Jan 26, 2021·Access Microbiology·Rachael NewtonJoyce E Patrick
Aug 28, 1999·Journal of Bacteriology·B A LazazzeraA D Grossman
Jul 1, 1992·Journal of Bacteriology·J P Mueller, A L Sonenshein
Apr 1, 1997·Journal of Bacteriology·F J GrundyT M Henkin
Nov 1, 1990·Journal of Bacteriology·F J Grundy, T M Henkin
May 1, 1993·Journal of Bacteriology·M M Nakano, P Zuber

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