Cloning and characterization of the 5'-flanking sequence for the human DNA topoisomerase II beta gene

Gene
S W NgL E Schnipper

Abstract

Mammalian cells express two isoforms of type II DNA topoisomerase which are the intracellular targets of many structurally diverse antineoplastic agents. The levels of topoisomerase II isozymes are important determinants for the sensitivity of cells to the cytotoxicity of drugs that target topoisomerase II. To investigate whether the expression of topoisomerase II isoforms is coordinated and the mechanisms governing their expression in the context of drug resistance, the 5'-flanking sequence for the gene of human topoisomerase IIbeta isoform was cloned and characterized. The 5'-flanking region has a very high GC content and contains no canonical TATA box element. Two separate transcriptional start sites are located to an adenine and a guanine, 193 and 89 nucleotides, respectively, upstream from the ATG translation initiation codon. Except for a small region immediately upstream of the translation initiation codon, there is no obvious sequence homology between the 5'-flanking sequences of human topoisomerase IIbeta gene and the previously described alpha gene. Transient expression assays with different 5'- and 3'-deletions of the 5'-flanking region of the topoisomerase IIbeta gene have delineated regions important for transcript...Continue Reading

References

Apr 1, 1992·Current Opinion in Genetics & Development·U K LaemmliY Adachi
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B Y BuggD P Suttle
Sep 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·A LeaskE Fuchs
Jan 1, 1989·Annual Review of Biochemistry·L F Liu
Sep 1, 1982·Molecular and Cellular Biology·C M GormanB H Howard
Apr 14, 1995·The Journal of Biological Chemistry·X YangE S Deneris
Apr 15, 1994·International Journal of Cancer. Journal International Du Cancer·L K HoskingB T Hill
Aug 3, 1994·Journal of the National Cancer Institute·J P JaffrézouB I Sikic
Jan 1, 1993·Annual Review of Biochemistry·M M Gottesman, I Pastan
Jan 3, 1996·International Journal of Cancer. Journal International Du Cancer·S L DaviesJ R Masters
Mar 26, 2010·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Ming-Yen LinHerng-Chia Chiu

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Citations

Sep 19, 2018·International Journal of Molecular Sciences·Caroline A AustinIan G Cowell
Jul 6, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·S E MirskiS P Cole
Aug 11, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·William T. BeckUppoor G. Bhat

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