Cloning and characterization of the calreticulin gene in Asian seabass (Lates calcarifer)

Animal : an International Journal of Animal Bioscience
Z Y BaiG H Yue

Abstract

Calreticulin (CRT) is a Ca2+-binding molecular chaperone in the endoplasmic reticulum. We cloned and characterized the CRT gene in an important marine food fish species Asian seabass (Lates calcarifer). The full-length DNA of the CRT gene was 2194 bp, including a complete open reading frame encoding 420 amino acid residues, a 113 bp 5'-untranslated region and an 818 bp 3'-untranslated region. The CRT gene contained nine exons and eight introns covering a total of 2772 bp genomic DNA from the start to stop codon. Ten single nucleotide polymorphisms (SNPs) were detected in introns and an exon in six individuals collected from five different locations. The CRT gene was assigned to linkage group 4 of the linkage map of Asian seabass. Quantitative real-time PCR revealed that the CRT gene was highly expressed in liver at the age of 1, 3 and 7 months under normal conditions, whereas its expression in liver reduced sharply after 0.5 to 2 h cold challenge at 16°C, and then increased slowly. A preliminary association analysis showed a significant (P < 0.001) association between the SNP6 in the CRT gene and the mortality after cold challenge at 16°C. Our results suggest that the CRT gene is associated with cold tolerance of Asian seabass ...Continue Reading

References

Aug 11, 1997·The Journal of Cell Biology·M WaserM Michalak
Nov 24, 1999·The Biochemical Journal·M MichalakM Opas
Sep 24, 2002·Experimental Parasitology·Carlos Alexandre Sanchez FerreiraAoi Masuda
Feb 24, 2006·The Journal of Experimental Medicine·Simona PorcelliniFabio Grassi
Dec 21, 2006·Genetics·Chun Ming WangGen Hua Yue

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