PMID: 8585086Sep 1, 1995Paper

Cloning and characterization of the cDNAs encoding Na+ channel-specific toxins 1 and 2 of the scorpion Centruroides noxius Hoffmann

Toxicon : Official Journal of the International Society on Toxinology
A VázquezB Becerril

Abstract

Using a cDNA library prepared from venomous glands of the Mexican scorpion Centruroides noxius Hoffmann the genes that encode toxins 1 and 2 were identified, cloned and sequenced. In view of the proposed mechanism for processing the mature peptides coded by these two genes, the corresponding peptide-toxins were sequenced de novo. Mass spectrometric and 1H-NMR analyses of the C-terminal peptide produced by enzymatic digestion of both toxins indicated that the last residue is serine-amide. Sequence comparison revealed that these two genes have a similarity of 56% and 80% at the amino acid and nucleotide levels, respectively. Small corrections to the published primary structures were introduced: Cn toxin 1 has an extra serine residue at position 65 and the residue in position 60 is a proline, while the amino acids at positions 34 and 35 of Cn 2 are, respectively, tyrosine and glycine. Sequence comparison of toxins from the genus Centruroides suggests the presence of at least three classes of distinct peptides in these venoms.

References

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Citations

Aug 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·A F LiceaL D Possani
Feb 6, 2004·Toxicon : Official Journal of the International Society on Toxinology·Jianbo DiaoSongping Liang
Jun 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·B BecerrilL D Possani
Jul 1, 1997·Toxicon : Official Journal of the International Society on Toxinology·R Ben KhalifaD Mebs
Dec 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·E KalapothakisV F Prado
May 10, 2001·Archives of Medical Research·Y Y Pérez-TorresJ Tortoriello-García
Mar 1, 1997·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C GarcíaL D Possani
Sep 22, 1999·European Journal of Biochemistry·L D PossaniJ Tytgat
Jul 11, 2013·PloS One·Laura L Valdez-VelázquezLourival D Possani
Nov 30, 2012·Biopolymers·Elisabeth F SchwartzMárcia R Mortari
Jul 31, 2013·Toxicon : Official Journal of the International Society on Toxinology·V Quintero-HernándezL D Possani
Oct 8, 2011·Toxicon : Official Journal of the International Society on Toxinology·V Quintero-HernándezL D Possani
Jan 2, 2007·Toxicon : Official Journal of the International Society on Toxinology·Michael GurevitzDalia Gordon
Dec 17, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Adolfo BorgesHuub J M Op den Camp
Jul 16, 2014·Toxins·Sebastián Estrada-GómezLeidy Johana Vargas Muñoz
Oct 16, 2001·Toxicon : Official Journal of the International Society on Toxinology·M CoronaL D Possani
Apr 5, 2012·The Journal of Physical Chemistry. B·Rong Chen, Shin-Ho Chung
Nov 18, 2019·Toxicon : Official Journal of the International Society on Toxinology·I A García-GuerreroL D Possani

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