Cloning and characterization of the full-length cDNA and genomic sequences encoding murine acid ceramidase

Genomics
C M LiE H Schuchman

Abstract

The full-length cDNA and genomic sequences encoding murine acid ceramidase (AC; E.C. 3.5.1.23) have been isolated and characterized. The 2176-bp cDNA was approximately 80% identical to the human cDNA (Koch et al., 1996) and predicted a 394-amino-acid polypeptide that was approximately 90% identical to the human protein. A fluorescence-based assay system was developed to determine AC enzymatic activity, and transfection of COS-1 cells with the full-length mouse cDNA led to increased AC activity, demonstrating its functionality. The murine AC gene, which spanned approximately 38 kb, consisted of 14 exons separated by 13 introns. The exons ranged in size from 46 to 1038 bp and were flanked by exon/intron junctions that adhered closely to known donor and acceptor splice site consensus sequences. Exon 1 encoded the putative translation start site and the signal peptide region, while exon 14 encoded the carboxy end of the AC polypeptide and all of the 3' untranslated region. Sequence analysis of a 497-bp region upstream from the first in-frame ATG revealed several features of a housekeeping promoter, as well as several tissue-specific and/or hormone-inducible regulatory sites. Insertion of this sequence into a chloramphenicol acyltra...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Nov 30, 1992·Biochemical and Biophysical Research Communications·J RotherK Sandhoff
Jun 11, 1986·Nucleic Acids Research·G von Heijne
May 12, 1995·The Journal of Biological Chemistry·K BernardoK Sandhoff
Apr 1, 1996·Current Opinion in Cell Biology·S SpiegelR Kolesnick

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Citations

Apr 16, 2011·The Journal of Biological Chemistry·Natasha C Lucki, Marion B Sewer
Apr 2, 2011·Antioxidants & Redox Signaling·Mariana N Nikolova-Karakashian, Michael B Reid
Apr 15, 2011·Journal of Lipids·Denny ProkschChristoph Arenz
Aug 3, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Efrat EliyahuEdward H Schuchman
Mar 10, 2010·Journal of Lipid Research·You-Hai XuGregory A Grabowski
Jun 13, 2008·The Review of Diabetic Studies : RDS·Marek Straczkowski, Irina Kowalska
Dec 12, 2012·Proceedings of the Japan Academy. Series B, Physical and Biological Sciences·Konrad Sandhoff
Sep 26, 2013·Biochimica Et Biophysica Acta·Makoto ItoMotohiro Tani
Sep 20, 2012·Bioorganic & Medicinal Chemistry·Krishna P BhabakChristoph Arenz
Sep 4, 2012·Prostaglandins & Other Lipid Mediators·Ashley J SniderLina M Obeid
Apr 9, 2010·Kidney International·Ankathrin FörsterJosef Pfeilschifter
Aug 6, 2008·Journal of Cellular Biochemistry·Agnieszka Błachnio-ZabielskaJan Górski
May 28, 2011·British Journal of Pharmacology·Daniel CanalsYusuf A Hannun
May 3, 2011·Archives of Biochemistry and Biophysics·Sean M O'NeillMark Kester
Feb 28, 2006·Biochimica Et Biophysica Acta·Jae-Ho ParkEdward H Schuchman
Oct 27, 2006·Biochimica Et Biophysica Acta·Jae-Ho Park, Edward H Schuchman
Sep 20, 2000·Molecular Genetics and Metabolism·Z ZhangA B Mukherjee
Aug 26, 2000·Genes, Chromosomes & Cancer·R S SeelanW Liu
Jun 17, 2018·Biological Chemistry·Nadine BeckmannAlexander Carpinteiro
Mar 19, 2005·The Journal of Biological Chemistry·Jose Antonio ChavezScott A Summers
Dec 29, 2005·The Journal of Biological Chemistry·Mari KonoRichard L Proia
Feb 1, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Efrat EliyahuEdward H Schuchman
Apr 1, 2019·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Justa Friebus-KardashMelanie Fiedler
Aug 3, 2014·EMBO Molecular Medicine·Yael Pewzner-JungErich Gulbins
Apr 5, 2000·The Journal of Biological Chemistry·O CuvillierS Spiegel
May 24, 2003·The Journal of Biological Chemistry·Nozomu OkinoEdward H Schuchman
May 6, 2015·Biological Chemistry·Michael FrohberghEdward H Schuchman

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