Cloning and Characterization of the Gene Cluster Involved in the Production of the Circular Bacteriocin Carnocyclin A

Probiotics and Antimicrobial Proteins
M J Van BelkumJ C Vederas

Abstract

Carnocyclin A is a circular bacteriocin of 60 amino acids produced by Carnobacterium maltaromaticum UAL307. A region of 12 kb that contained the structural gene for carnocyclin A, cclA, was sequenced using a fosmid library, and 10 genes were identified that could be responsible for carnocyclin A production and immunity. Five of those genes, cclBITCD, were found upstream of cclA: one encodes a protein containing a conserved ATP-binding domain and four encode proteins with putative membrane-spanning domains. CclC shows homology with a family of membrane proteins that contain the domain of unknown function 95 (DUF95). Downstream of cclA four additional genes, cclEFGH, were identified that show similarity to the last four genes, as-48EFGH, of the enterocin AS-48 bacteriocin gene cluster. CclFGH shows sequence homology with As-48FGH. Transformation of C. maltaromaticum UAL26 with cclBITCDA resulted in production of carnocyclin A, indicating that these genes form the minimal requirement for the secretion of fully matured bacteriocin. cclI encodes for a small hydrophobic protein with a high pI, which are characteristic features of known immunity proteins for other circular bacteriocins. Indeed, cloning of cclI behind a constitutive pr...Continue Reading

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Citations

Dec 1, 2012·Probiotics and Antimicrobial Proteins·Marco J van Belkum, John C Vederas
Jun 15, 2011·Trends in Microbiology·Marco J van BelkumJohn C Vederas
Feb 22, 2012·Beneficial Microbes·Y MasudaK Sonomoto
Feb 15, 2015·Applied and Environmental Microbiology·Jeella Z AcedoJohn C Vederas
Aug 31, 2014·Applied and Environmental Microbiology·Christina GabrielsenDzung B Diep
Feb 28, 2014·Microbiology·Amina PotterMaria do Carmo de Freire Bastos
Sep 21, 2018·Frontiers in Microbiology·Rodney H PerezKenji Sonomoto
Mar 22, 2018·Applied Microbiology and Biotechnology·Sen Zheng, Kenji Sonomoto

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