PMID: 9177484May 2, 1997Paper

Cloning and characterization of the gene encoding a mitochondrially localized DNA topoisomerase II in Dictyostelium discoideum. Western blot analysis

Biochimica Et Biophysica Acta
K KomoriY Tanaka

Abstract

We cloned a gene (topA) encoding DNA topoisomerase II from Dictyostelium discoideum nuclear DNA using oligo probes corresponding to the consensus amino acid sequences found in the gene in other eukaryotes. The gene encoding a predicted polypeptide of 1282 amino acids with M(r) of about 146 kDa, is a single copy that is expressed as a polyadenylated 4.5 kb RNA. The predicted amino acid sequence shares similarity with those of other eukaryotes with identity between 32 and 46%. The protein is 260-300 amino acids shorter in the C-terminal region and 50-80 longer in the N-terminal region than those of other eukaryotes. In TopA of D. discoideum, the N-terminal region with stretches of charged and hydrophilic amino acids is predicted to fold into an amphiphilic alpha-helix which is characteristic of a mitochondrial targeting signal presequence. Four independent polyclonal antibodies against bacterially expressed GST fusion proteins containing four portions of the polypeptide detected a single band on Western blots at about 135 kDa. Western blots analysis of subcellular fractions revealed that this protein is localized in mitochondria. The protein and the mRNA are present in growth phase and during development, although levels of both ...Continue Reading

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Jun 1, 1992·Virology·R García-BeatoM L Salas
Jun 1, 1992·Current Opinion in Cell Biology·T S Hsieh
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A Kuspa, W F Loomis
Dec 1, 1992·The Journal of Cell Biology·K Shiozaki, M Yanagida
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M R Gartenberg, J C Wang
Dec 1, 1991·Molecular and Cellular Biology·K Shiozaki, M Yanagida
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J E Lindsley, J C Wang
Apr 1, 1991·Developmental Biology·D L RichardsonW F Loomis
Jan 18, 1989·Biochimica Et Biophysica Acta·F U HartlW Neupert
Oct 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M Tsai-PflugfelderJ C Wang
Jul 25, 1988·Nucleic Acids Research·H M Warrick, J A Spudich
May 1, 1970·The Journal of Cell Biology·S M Cocucci, M Sussman
Feb 1, 1971·Experimental Cell Research·W F Loomis
Jul 1, 1966·Genetics·M DemerecP E Hartman
Jan 1, 1983·Methods in Enzymology·D Hanahan, M Meselson
May 10, 1995·Archives of Biochemistry and Biophysics·M A Birney, C Klein
Nov 1, 1994·The Biochemical Journal·P M Watt, I D Hickson
May 1, 1994·Molecular and Cellular Biology·P R CaronJ C Wang
Jun 1, 1993·Molecular and Cellular Biology·D Rose, C Holm
Jan 1, 1996·Annual Review of Biochemistry·J C Wang
Sep 1, 1995·Trends in Cell Biology·L Poljak, E Käs

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Citations

May 30, 2001·Advanced Drug Delivery Reviews·T C RoweJ W Lawrence
Oct 2, 2003·European Journal of Biochemistry·Robert L LowDavid B Friedman
Sep 5, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yong WangJames C Wang
Aug 4, 2016·Nucleic Acids Research·Yicheng LongJane E Jackman
Jun 21, 2018·Annual Review of Microbiology·Gareth Bloomfield
Feb 9, 2006·Chemical Reviews·Maria A GraziewiczWilliam C Copeland
Mar 17, 1999·Biochimica Et Biophysica Acta·J W Taanman

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