Cloning and characterization of the grass carp (Ctenopharyngodon idella) Toll-like receptor 22 gene, a fish-specific gene

Fish & Shellfish Immunology
Jianjian LvYaping Wang

Abstract

Toll-like receptor 22 (TLR22) is a fish-specific TLR which recognizes double-strand (ds) RNA and participates in the innate immune response through the Toll-IL-1R homology domain-containing adaptor protein 1 (TICAM-1). To further investigate how the innate immune system of teleosts responds to viral infections, we cloned the full-length cDNA sequence of grass carp (Ctenopharyngodon idella) TLR22 (CiTLR22). The complete cDNA sequence of CiTLR22 was 3647 bp and encodes a polypeptide of 954 amino acids. Analysis of the deduced amino acid sequence indicated that CiTLR22 has typical structural features of proteins belonging to the TLR family. These included 17 LRR domains (residues 88-634) and one C-terminal LRR domain (LRR-CT, residues 694-745) in the extracellular region, and a TIR domain (residues 801-944) in the cytoplasmic region. Comparison with homologous proteins showed that the deduced CiTLR22 has the highest sequence identity to common carp TLR22 (82.9%). Genomic DNA of CiTLR22 was obtained by long-distance (Ld) PCR and structure analysis revealed that the CiTLR22 gene is encoded by uninterrupted exons. Reverse transcriptase-PCR (RT-PCR) revealed that CiTLR22 is a non-maternal gene. It is prominently expressed in immune re...Continue Reading

References

Nov 1, 1996·Scientific American·G W Litman
Jan 1, 1996·Annual Review of Cell and Developmental Biology·M P Belvin, K V Anderson
Jan 29, 2002·Nature Immunology·Jules A Hoffmann, Jean-Marc Reichhart
Apr 26, 2002·Current Opinion in Immunology·Gregory M Barton, Ruslan Medzhitov
Dec 13, 2002·Proceedings of the National Academy of Sciences of the United States of America·Robert L StrausbergUNKNOWN Mammalian Gene Collection Program Team
Dec 24, 2002·Trends in Immunology·Geoffrey B JohnsonJeffrey L Platt
Dec 24, 2002·Current Opinion in Immunology·Shizuo Akira
Jun 12, 2003·Developmental and Comparative Immunology·James L StaffordBrad G Magor
Sep 19, 2003·Genomics·Andrew K JonesDavid B Sattelle
Dec 23, 2003·Molecular Immunology·Cyril JaultJohanna Chluba
Dec 23, 2003·Molecular Immunology·Annemarie H MeijerHerman P Spaink
Apr 6, 2004·Developmental and Comparative Immunology·Sumathi SubramaniamCharles Cunningham
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Dec 31, 2005·Nucleic Acids Research·Ivica LetunicPeer Bork
Nov 23, 2006·Fish & Shellfish Immunology·Puttharat BaoprasertkulZhanjiang Liu
Apr 26, 2007·Nature Reviews. Immunology·Luke A J O'Neill, Andrew G Bowie
Jan 6, 2009·Immunological Reviews·Tsukasa SeyaHiroyuki Oshiumi
Jun 10, 2009·Current Genomics·Hiroyuki OshiumiTsukasa Seya
Oct 24, 2009·Veterinary Immunology and Immunopathology·Alexander ReblHans-Martin Seyfert
Aug 17, 2010·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Mahmoud TanekhyMasahiro Sakai
Feb 20, 2016·Neuroscience and Biobehavioral Reviews·Susana Aznar, Mona El-Sayed Hervig

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Citations

Mar 7, 2013·Fish & Shellfish Immunology·Takashi AokiTae Sung Jung
Oct 2, 2013·Fish & Shellfish Immunology·Gustavo Núñez-Acuña, Cristian Gallardo-Escárate
Jan 1, 2013·BMC Evolutionary Biology·Arvind Y M SundaramJorge M O Fernandes
Sep 23, 2014·Fish & Shellfish Immunology·Jie ZhangXuejun Li
Jul 18, 2015·Biology·Sarah PoynterStephanie DeWitte-Orr
Feb 26, 2019·Frontiers in Immunology·Chao-Yang LaiTsung-Hsien Chuang

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