PMID: 7764570Feb 1, 1994Paper

Cloning and characterization of the hemA gene for synthesis of delta-aminolevulinic acid in Xanthomonas campestris pv. phaseoli

Applied Microbiology and Biotechnology
N AsaharaY Murooka

Abstract

The gene from Xanthomonas campestris pv. phaseoli that is involved in the C5 pathway of delta-amino-levulinic acid (ALA) of Escherichia coli. Subcloning of deletion fragments from the initial 2.5-kilobase (kb) chromosomal fragment allowed the isolation of a 1.6-kb fragment that could complement the hemM mutation. Nucleotide sequence analysis of the 1.6-kb DNA fragment revealed an open reading frame that encodes a polypeptide of 426 amino acid residues, and the deduced molecular mass of this polypeptide is 46768 Da. The amino acid sequence shows a high degree of homology of the HemA protein, which is glutamyl-tRNA reductase, to other organisms. Thus, we examined the complementation test of the cloned gene from Xanthomonas with a hemA mutation of E. coli and found that the gene complemented the hemA mutation. These results suggest that the cloned gene is hemA and the gene from Xanthomonas also complements both hemA and hemM mutations, as in the case of the E. coli hemA.

Citations

Jun 1, 1995·Photosynthesis Research·Y J Avissar, P A Moberg
Aug 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·U C VothknechtD von Wettstein
Sep 4, 2001·Biotechnology & Genetic Engineering Reviews·S Nishikawa, Y Murooka

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