Cloning and characterization of the human Calmegin gene encoding putative testis-specific chaperone

Gene
H TanakaY Nishimune

Abstract

The putative chaperone Calmegin is required for sperm fertility in mouse and the relevance of the gene to certain cases of human male infertility has been suggested. In the present paper, we have isolated and characterized the human homolog cDNA of the mouse germ cell-specific Calmegin. The entire coding region of the human cDNA showed 80% identity with the previously reported mouse Calmegin. The predicted amino acid sequence showed strong conservation of the two sets of internal repetitive sequences (Ca2+ binding motif), and the hydrophilic COOH terminus, which corresponds to the putative endoplasmic reticulum (ER) retention motif. Our finding will support diagnosis of male infertility. Northern blotting analysis of various human tissues showed that the transcript was 3 kb in length and was expressed exclusively in the testis. Using the fluorescence in situ hybridization (FISH) technique, human Calmegin gene was mapped to chromosome 4q28.3-q31.1.

References

Nov 1, 1992·Molecular Reproduction and Development·D WatanabeY Nishimune
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·P LichterD C Ward
Mar 1, 1994·Trends in Biochemical Sciences·J J BergeronD B Williams
Feb 1, 1993·Fertility and Sterility·A MackennaI Cooke

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Citations

Oct 18, 2002·Gene·Staffan PerssonMarianne Sommarin
May 23, 2015·Asian Journal of Andrology·Ashok AgarwalEdmund Sabanegh
Mar 15, 2006·International Journal of Andrology·Hiromitsu TanakaYoshitake Nishimune
Sep 26, 2013·Developmental and Comparative Immunology·Lital SeverBrian Dixon
Jul 28, 2011·International Journal of Andrology·R Waheeb, M-C Hofmann
Jul 28, 2005·Reproductive Medicine and Biology·Yasuhiro MatsuokaHiromitsu Tanaka
Jul 27, 2005·Molecular and Cellular Endocrinology·Brett NixonR John Aitken

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