Cloning and characterization of the novel d-aspartyl endopeptidase, paenidase, from Paenibacillus sp. B38

Journal of Biochemistry
Satoru NirasawaSaori Takahashi

Abstract

Paenidase is the first micro-organism-derived d-aspartyl endopeptidase that specifically recognizes an internal d-Asp residue to cleave [d-Asp]-X peptide bonds. Using peptide sequences obtained from the protein, we performed PCR with degenerate primers to amplify the paenidase I-encoding gene. Nucleotide sequencing revealed that mature paenidase I consist of 322 amino acid residues and that the protein is encoded as a pro-protein with a 197-amino-acid N-terminal extension compared to the mature protein. Paenidase I exhibits amino acid sequence similarity to several penicillin-binding proteins. In addition, paenidase I was classified into peptidase family S12 based on a MEROPS database search. Family S12 contains serine-type d-Ala-d-Ala carboxypeptidases that have three active site residues (Ser, Lys and Tyr) in the conserved motifs Ser-Xaa-Thr-Lys and Tyr-Xaa-Asn. These motifs were conserved in the primary structure of paenidase I, and the role of these residues was confirmed by site-directed mutagenesis.

References

May 25, 1971·Biochemistry·M Leyh-BouilleM Nieto
Dec 28, 1971·Journal of Molecular Biology·J King, U K Laemmli
Mar 1, 1967·European Journal of Biochemistry·P Edman, G Begg
Jun 24, 1983·Science·E H ManG H Fisher
Sep 1, 1995·The Plant Journal : for Cell and Molecular Biology·Y G LiuR F Whittier
Nov 22, 1996·The Journal of Biological Chemistry·Y AsanoY Kato
Jun 22, 2002·The Journal of Biological Chemistry·Jean-Luc MainardiLaurent Gutmann
Nov 26, 2002·Journal of Molecular Biology·Loredano PollegioniMirella S Pilone
Feb 7, 2003·Applied and Environmental Microbiology·Dae Heoun BaekMoon-Hee Sung
Sep 6, 2005·Biological & Pharmaceutical Bulletin·Noriko Fujii
Aug 17, 2006·FEMS Microbiology Reviews·Pauline MacheboeufAndréa Dessen
Dec 18, 2007·Applied Microbiology and Biotechnology·Loredano PollegioniMirella S Pilone
Sep 29, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Tadatoshi KinouchiNoriko Fujii

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