Cloning and characterization of the promoter of human Wnt inhibitory factor-1

Biochemical and Biophysical Research Communications
Noemi ReguartDavid M Jablons

Abstract

Wnt inhibitory factor-1 (WIF-1) is a secreted antagonist of Wnt signaling and functions by directly binding to Wnt ligands in the extracellular space. Here we report the identification of the 5' promoter region (approximately 1.5 kb) of the human WIF-1 gene. Functional analysis of this region shows that a whole fragment displays high basal promoter activity in different cell lines, while the truncated forms do not, indicating that integrity of the WIF-1 promoter region may be important for WIF-1 activity. Moreover, we found that the expression level of beta-catenin in cancer cell lines correlates with the WIF-1 promoter activity, suggesting that the WIF-1 promoter may be regulated by the Wnt/beta-catenin pathway and may function in a negative feedback manner. Our results also suggest that a methylated CpG island, which we observed recently in human lung cancer, lies within the functional WIF-1 promoter region and therefore bears the importance of the methylation-status of this CpG island as an important key in Wnt activation in human cancer.

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Citations

Aug 11, 2010·Oncogene·J D F LicchesiJ G Herman
Jan 24, 2006·Biochemical and Biophysical Research Communications·Yu-Ching LinDavid M Jablons
Mar 7, 2006·Biochemical and Biophysical Research Communications·Sonny BatraDavid M Jablons
Aug 25, 2009·The American Journal of Pathology·Andrey A SharovVladimir A Botchkarev
Mar 26, 2010·Journal of Experimental & Clinical Cancer Research : CR·Zhuanyi YangYanjin Wang
Aug 29, 2014·Integrative Biology : Quantitative Biosciences From Nano to Macro·Shriprakash Sinha
Nov 2, 2016·Applied Immunohistochemistry & Molecular Morphology : AIMM·Yue Zhang, Chunhong Hu
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Dec 24, 2018·Frontiers in Cell and Developmental Biology·Lucia PoggiMatthias Carl
Jan 21, 2020·Cancer Biomarkers : Section a of Disease Markers·Lirong WuXia He
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Apr 3, 2020·Scientific Reports·Hui ZhangYong Liang
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Shinji UrakamiRajvir Dahiya

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