PMID: 9537370Apr 16, 1998Paper

Cloning and characterization of the Pseudomonas aeruginosa zwf gene encoding glucose-6-phosphate dehydrogenase, an enzyme important in resistance to methyl viologen (paraquat)

Journal of Bacteriology
J F MaD J Hassett

Abstract

In this study, we cloned the Pseudomonas aeruginosa zwf gene, encoding glucose-6-phosphate dehydrogenase (G6PDH), an enzyme that catalyzes the NAD+- or NADP+-dependent conversion of glucose-6-phosphate to 6-phosphogluconate. The predicted zwf gene product is 490 residues, which could form a tetramer with a molecular mass of approximately 220 kDa. G6PDH activity and zwf transcription were maximal in early logarithmic phase when inducing substrates such as glycerol, glucose, or gluconate were abundant. In contrast, both G6PDH activity and zwf transcription plummeted dramatically when bacteria approached stationary phase, when inducing substrate was limiting, or when the organisms were grown in a citrate-, succinate-, or acetate-containing basal salts medium. G6PDH was purified to homogeneity, and its molecular mass was estimated to be approximately 220 kDa by size exclusion chromatography. Estimated Km values of purified G6PDH acting on glucose-6-phosphate, NADP+, and NAD+ were 530, 57, and 333 microM, respectively. The specific activities with NAD+ and NADP+ were calculated to be 176 and 69 micromol/min/mg. An isogenic zwf mutant was unable to grow on minimal medium supplemented with mannitol. The mutant also demonstrated increa...Continue Reading

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Citations

Mar 19, 2013·PLoS Pathogens·Slavica DjonovićFrederick M Ausubel
Apr 21, 2005·Applied Microbiology and Biotechnology·Carla C C R de CarvalhoM Manuela R da Fonseca
Oct 24, 2002·FEMS Microbiology Letters·Lothar PetruschkaHeidrun Herrmann
Jan 20, 2004·Bioscience, Biotechnology, and Biochemistry·Naoto TonouchiKenzo Yokozeki
Feb 28, 2015·Journal of Industrial Microbiology & Biotechnology·Hongmiao PanHuizhong Chen
Mar 21, 2019·International Journal of Molecular Sciences·Kiet TranNgocSei-Heon Jang
Oct 3, 2002·Biochemical and Biophysical Research Communications·Paiboon VatanaviboonSkorn Mongkolsuk
Nov 30, 2004·Trends in Molecular Medicine·Gee W LauFansheng Kong

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