Cloning and characterization of the putative AFAP1-AS1 promoter region

Journal of Cancer
Fang WeiFang Xiong

Abstract

Actin filament-associated protein 1-antisense RNA1 (AFAP1-AS1), a cancer-related long non-coding RNA, has been found to be upregulated in multiple types of cancers. AFAP1-AS1 is important for the initiation, progression and poor prognosis of many cancers, including nasopharyngeal carcinoma (NPC). However, the mechanism underlying the regulation of AFAP1-AS1 expression is not well-understood. In our study, the potential promoter region of AFAP1-AS1 was predicted by comprehensive bioinformatics analysis. Moreover, promoter deletion analysis identified the sequence between positions -359 and -28 bp as the minimal promoter region of AFAP1-AS1. The ChIP assay results indicate that the AFAP1-AS1 promoter is responsive to the transcription factor c-Myc, which can promote high AFAP1-AS1 expression. This study is the first to clone and characterize the AFAP1-AS1 promoter region. Our findings will help to better understand the underlying mechanism of high AFAP1-AS1 expression in tumorigenesis and to develop new strategies for therapeutic high expression of AFAP1-AS1 in NPC.

Citations

May 3, 2019·Cancer Science·Chun-Mei FanZhao-Yang Zeng
Apr 20, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dan WangCan Guo
May 5, 2020·Molecular Oncology·Ting TangZhaoyang Zeng
Feb 3, 2021·Photodiagnosis and Photodynamic Therapy·Haixiu MaLing Kang

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Methods Mentioned

BETA
PCR
transfection
immunoprecipitation
electrophoresis
GTPase

Software Mentioned

CpGProD
CpG Island Searcher
BDGP
CpG Islands
GraphPad Prism

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