PMID: 2507522Oct 1, 1989Paper

Cloning and characterization of the regulatory Bacillus subtilis competence genes comA and comB

Journal of Bacteriology
N GuillenD A Dubnau

Abstract

comA and comB are Bacillus subtilis competence genes that are identified by insertions of Tn917lac. They are classified as early genes because of their expression throughout growth; the expression of late com genes increases sharply during the transition to the stationary phase. The comA and comB determinants were cloned, and the 5' and 3' termini of their transcripts were localized by low-resolution S1 nuclease protection experiments. comA and comB were found by Southern blotting to be localized near one another, but they were nevertheless apparently transcribed independently. Epistatic relationships among the com genes were explored by using the beta-galactosidase expressed from transcriptional fusions as a marker. Late com genes were found to be dependent on the products of comA, comB, and sin for their expression. The sin gene is a transcriptional regulator that is required for the development of competence (N. K. Gaur, E. Dubnau, and I. Smith, J. Bacteriol. 168:860-869, 1986).

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Citations

Dec 7, 2010·Journal of Bacteriology·Kathryn J PflughoeftTheresa M Koehler
Apr 20, 2011·Letters in Applied Microbiology·R V VelhoA Brandelli
Jan 25, 2006·Molecular Microbiology·Frances ChuRichard Losick
Jan 1, 1991·Molecular Microbiology·D Dubnau
Jan 1, 1990·Critical Reviews in Biotechnology·M M Nakano, P Zuber
Aug 20, 2020·Applied Microbiology and Biotechnology·Rongrong YangJunling Shi
Sep 5, 2006·Journal of Bacteriology·Christian RossEduardo Robleto
Sep 11, 2007·Applied and Environmental Microbiology·Alexandra KoumoutsiRainer Borriss
Jan 26, 2021·Access Microbiology·Rachael NewtonJoyce E Patrick
May 1, 1992·Journal of Bacteriology·A Raymond-Denise, N Guillen
Oct 1, 1989·Journal of Bacteriology·M AlbanoD A Dubnau
Oct 1, 1996·Journal of Bacteriology·A J Dijkstra, W Keck
Oct 1, 1996·Journal of Bacteriology·P Serror, A L Sonenshein
Apr 1, 1994·Journal of Bacteriology·L M Márquez-MagañaM J Chamberlin
Jul 1, 1990·Journal of Bacteriology·D Dubnau, M Roggiani
Jul 1, 1990·Journal of Bacteriology·M RoggianiD Dubnau
Sep 1, 1991·Microbiological Reviews·D Dubnau
Nov 1, 1991·Journal of Bacteriology·A Raymond-Denise, N Guillen
Jun 30, 2007·BMC Genomics·Hirokazu KobayashiShigehiko Kanaya

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