Cloning and characterization of the vitamin D receptor from Xenopus laevis

Endocrinology
Y C LiM B Demay

Abstract

The Vitamin D receptor (VDR), a member of the nuclear receptor superfamily, mediates the effects of 1,25-dihydroxyvitamin D3 on mineral ion homeostasis. Although the mammalian and avian VDRs have been extensively studied, little is known about the VDR in lower vertebrate species. To address this, we have isolated the Xenopus laevis VDR (xVDR) complementary DNA. Overall, the xVDR shares 79%, 73%, 73%, and 75% identity at the amino acid level with the chicken, mouse, rat, and human VDRs, respectively. The amino acid residues and subdomains important for DNA binding, hormone binding, dimerization, and transactivation are mostly conserved among all VDR species. The xVDR polypeptide can heterodimerize with the mouse retinoid X receptor alpha, bind to the rat osteocalcin vitamin D response element (VDRE), and induce vitamin D-dependent transactivation in transfected mammalian cells. Northern analysis reveals two xVDR messenger RNA species of 2.2 kb and 1.8 kb in stage 60 Xenopus tissues. In the adult, xVDR expression is detected in many tissues including kidney, intestine, skin, and bone. During Xenopus development, xVDR messenger RNA first appears at developmental stage 13 (pre-neurulation), increasing to maximum at stages 57-61 (me...Continue Reading

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Citations

Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y C LiM B Demay
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·F Jehan, H F DeLuca
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·L A CroftsJ A Eisman
Mar 20, 2010·BMC Evolutionary Biology·Sylvain MarcelliniMartín Montecino
Nov 14, 2007·BMC Evolutionary Biology·Erica J ReschlyMatthew D Krasowski
May 19, 2012·Endocrine Reviews·Clifford J RosenChristopher S Kovacs
Feb 9, 2008·Endocrinology·Deanna L HowarthSeth W Kullman
Mar 23, 2011·Respiratory Research·Christian HerrRobert Bals
Jul 12, 2012·Calcified Tissue International·Mark R HausslerPeter W Jurutka
Sep 12, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M L CancelaP A Price
Jul 4, 2012·Protein Expression and Purification·Jinge ZhuFariba M Assadi-Porter
Sep 13, 2008·The FEBS Journal·Etienne RicherMathieu F M Cellier
Oct 22, 2008·Biochemical and Biophysical Research Communications·Hideaki NagaokaMitsuo Yamauchi
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Sep 6, 2003·International Journal of Dermatology· Sultan Al-Khenaizan, Patricia Vitale

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