PMID: 9164846May 15, 1997Paper

Cloning and characterization of two glutathione S-transferases from a DDT-resistant strain of Anopheles gambiae

The Biochemical Journal
Hilary RansonJanet Hemingway

Abstract

Two cDNA species, aggst1-5 and aggst1-6, comprising the entire coding region of two distinct glutathione S-transferases (GSTs) have been isolated from a 1,1,1-trichloro-2,2-bis-(p-chlorophenyl)ethane (DDT) resistant strain (ZANDS) of Anopheles gambiae. The nucleotide sequences of these cDNA species share 80.2% identity and their derived amino acid sequences are 82.3% similar. They have been classified as insect class I GSTs on the basis of their high sequence similarity to class I GSTs from Drosophila melanogaster and Musca domestica and they are localized to a region of an An. gambiae chromosome known to contain further class I GSTs. The genes aggst1-5 and aggst1-6 were expressed at high levels in Escherichia coli and the recombinant GSTs were purified by affinity chromatography and characterized. Both agGST1-5 and agGST1-6 showed high activity with the substrates 1-chloro-2,4-dinitrobenzene and 1, 2-dichloro-4-nitrobenzene but negligible activity with the mammalian theta class substrates, 1,2-epoxy-3-(4-nitrophenoxy)propane and p-nitrophenyl bromide. Despite their high level of sequence identity, agGST1-5 and agGST1-6 displayed different kinetic properties. Both enzymes were able to metabolize DDT and were localized to a subs...Continue Reading

Citations

Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·H RansonJ Hemingway
Apr 13, 2000·Annual Review of Entomology·J Hemingway, H Ranson
Jun 15, 2011·Journal of Medical Entomology·K GunasekaranP Jambulingam
Aug 9, 2007·Marine Biotechnology·Brian BlanchetteBal Ram Singh
Aug 24, 2006·Annual Review of Entomology·Xianchun LiMay R Berenbaum
Feb 18, 1999·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·J HemingwayH Ranson
Jan 16, 2010·Archives of Insect Biochemistry and Physiology·Ruchita SelotPradeep G Kumar
Sep 17, 2009·Annual Review of Entomology·A Enayati, J Hemingway
Mar 1, 2016·Biochemistry Research International·Patience MarimoStanley Mukanganyama
Aug 4, 2020·Journal of Molecular Biology·Madhuranayaki Thulasingam, Jesper Z Haeggström
Sep 16, 2000·Insect Biochemistry and Molecular Biology·J Hemingway
Jul 6, 2001·Insect Biochemistry and Molecular Biology·K JirajaroenratA J Ketterman
Aug 16, 2001·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Lesley I. McLellan, C. Roland Wolf
Jun 24, 2003·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Danyelle M TownsendHaim Tapiero
Nov 1, 2003·Archives of Biochemistry and Biophysics·Erminia CarlettiCarmine Di Ilio
Dec 31, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Nicolas VanhaelenEric Haubruge
Jun 10, 2005·Insect Biochemistry and Molecular Biology·Nongkran LumjuanHilary Ranson
Jun 14, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kohji YamamotoHiroshi Fujii
Jan 15, 2019·Journal of Agricultural and Food Chemistry·Wei WangXue-Qing Yang

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