PMID: 8990302Jan 1, 1997Paper

Cloning and characterization of two groESL operons of Rhodobacter sphaeroides: transcriptional regulation of the heat-induced groESL operon

Journal of Bacteriology
W T LeeF R Tabita

Abstract

The nonsulfur purple bacterium Rhodobacter sphaeroides was found to contain two groESL operons. The groESL1 heat shock operon was cloned from a genomic library, and a 2.8-kb DNA fragment was sequenced and found to contain the groES and groEL genes. The deduced amino acid sequences of GroEL1 (cpn60) and GroES1 (cpn10) were in agreement with N-terminal sequences previously obtained for the isolated proteins (K. C. Terlesky and F. R. Tabita, Biochemistry 30:8181-8186, 1991). These sequences show a high degree of similarity to groESL genes isolated from other bacteria. Northern analysis indicated that the groESL1 genes were expressed as part of a 2.2-kb polycistronic transcript that is induced 13-fold after heat shock. Transcript size was not affected by heat shock; however, the amount of transcript was induced to its greatest extent 15 to 30 min after a 40 degrees C heat shock, from an initial temperature of 28 degrees C, and remained elevated up to 120 min. The R. sphaeroides groESL1 operon contains a putative hairpin loop at the start of the transcript that is present in other bacterial heat shock genes. Primer extension of the message showed that the transcription start site is at the start of this conserved hairpin loop. In th...Continue Reading

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Citations

Apr 15, 2005·Archives of Microbiology·F Rodríguez-QuiñonesP A Lund
Sep 14, 2006·Molecular Microbiology·Georgia KapataiPeter A Lund
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Gregory W Clark, Elisabeth R M Tillier
Mar 17, 2010·Applied and Environmental Microbiology·Brian WitteF Robert Tabita
Jun 6, 1998·Journal of Bacteriology·J Zeilstra-RyallsS Kaplan
Jun 5, 2003·Microbiology·Dorota Kuchanny-Ardigò, Barbara Lipińska
Jan 9, 1998·Journal of Bacteriology·R K KarlsT J Donohue
Sep 21, 2001·Journal of Molecular Biology·T A FukamiK Miki
Dec 5, 1998·Microbiology and Molecular Biology Reviews : MMBR·S C BakerR J van Spanning
Oct 12, 2004·Biochemical and Biophysical Research Communications·Roger GeorgePeter A Lund

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