PMID: 9435065Jan 22, 1998Paper

Cloning and characterization of two pyruvate decarboxylase genes from Pichia stipitis CBS 6054

Applied and Environmental Microbiology
P LuT W Jeffries

Abstract

In Pichia stipitis, fermentative and pyruvate decarboxylase (PDC) activities increase with diminished oxygen rather than in response to fermentable sugars. To better characterize PDC expression and regulation, two genes for PDC (PsPDC1 and PsPDC2) were cloned and sequenced from P. stipitis CBS 6054. Aside from Saccharomyces cerevisiae, from which three PDC genes have been characterized, P. stipitis is the only organism from which multiple genes for PDC have been identified and characterized. PsPDC1 and PsPDC2 have diverged almost as far from one another as they have from the next most closely related known yeast gene. PsPDC1 contains an open reading frame of 1,791 nucleotides encoding 597 amino acids. PsPDC2 contains a reading frame of 1,710 nucleotides encoding 570 amino acids. An 81-nucleotide segment in the middle of the beta domain of PsPDC1 codes for a unique segment of 27 amino acids, which may play a role in allosteric regulation. The 5' regions of both P. stipitis genes include two putative TATA elements that make them similar to the PDC genes from S. cerevisiae, Kluyveromyces marxianus, and Hanseniaspora uvarum.

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Citations

Mar 2, 2002·Applied and Environmental Microbiology·Yong-Su JinThomas W Jeffries
Oct 29, 2008·Biotechnology Journal·Mark TaylorDon Cowan
Aug 8, 2009·FEMS Yeast Research·Thomas W Jeffries, Jennifer R Headman Van Vleet
Feb 21, 2013·Applied Microbiology and Biotechnology·Praveen Kumar AgarwalSantosh B Noronha
Oct 31, 2006·Applied and Environmental Microbiology·Marja IlménMerja Penttilä
Feb 13, 2001·Journal of Applied Microbiology·P BasaranR W Worobo
Oct 30, 2001·FEMS Microbiology Letters·F AmáritaC Pelaez
Apr 18, 1998·Applied and Environmental Microbiology·J Y Cho, T W Jeffries
Dec 15, 2020·FEMS Microbiology Reviews·Justyna Ruchala, Andriy A Sibirny
May 29, 1999·Applied and Environmental Microbiology·J Y Cho, T W Jeffries

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