Cloning and characterization of ZNF189, a novel human Krüppel-like zinc finger gene localized to chromosome 9q22-q31

Genomics
J OdebergJ Lundeberg

Abstract

A 3-kb-long cDNA encoding a Krüppel-like human zinc finger protein was isolated and mapped to chromosome 9q22-q31. The ZNF189 gene encodes a protein with 16 zinc fingers at its C-terminus and belongs to the Krüppel-associated box (KRAB)-containing group of zinc finger proteins. Four differently spliced cDNA transcripts, differing at the 5' coding region where a KRAB A repressor domain is encoded, were isolated. In addition, Northern blot analysis indicates the presence of two additional unidentified splice variants. Comparison of cDNA and genomic sequences shows that the ZNF189 gene spans approximately 11 kb and is organized into at least four exons, the large 3'-end exon coding for the complete zinc finger domain and the 3' untranslated region. ZNF189 is expressed in all tissues and cell types currently investigated, at varying levels, but with a tissue- or cell-type-restricted expression pattern for the different splice variants. ZNF189 is conserved in the genome of several mammalian species. Direct sequencing of the ZNF189 gene in microdissected tumor biopsies of sporadic basal cell carcinoma and squamous cell carcinoma reveals no mutations in the coding sequence or at exon/intron boundaries.

References

May 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·E J BellefroidJ A Martial
Jan 1, 1995·Human Molecular Genetics·S M ShanleyG Chenevix-Trench
Aug 15, 1996·Genes & Development·J R FriedmanF J Rauscher

❮ Previous
Next ❯

Citations

Jun 14, 2005·Biochemical and Biophysical Research Communications·Hong YangMingyao Liu
Jun 21, 2005·Biochemical and Biophysical Research Communications·Ying OuMingyao Liu
May 1, 2001·The Journal of Pathology·A A van TilborgE C Zwarthoff
Sep 8, 2004·Oecologia·Päivi LehtonenKari Saikkonen
Aug 21, 2019·Nature Neuroscience·Zachary S LorschEric J Nestler

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Carcinoma, Basal Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.