Cloning and developmental expression of the cytochrome P450 aromatase gene (CYP19) in the European eel (Anguilla anguilla)

General and Comparative Endocrinology
Itai TzchoriBoaz Moav

Abstract

To characterize the involvement of the aromatase gene during the process of sex determination in the European eel (Anguilla anguilla), the expression of its gonadal form was determined during various developmental stages. The cloned cDNA from the European eel gonad (EeCYP19) contains an open reading frame of 1539 bp, encoding a deduced protein of 513 residues. The predicted amino acid sequence shows 97% identity with that of the Japanese eel, and 59-69% of identity with those of the CYP19 gonadal and brain forms of other teleost fish. Two potential initiation sites (ATG) were found downstream of the first ATG codon. A fluorescent-based method of real-time PCR was developed to quantify EeCYP19 expression. The expression levels of EeCYP19 in the gonads of adult males were approximately 12- and 30-fold lower than the levels in adult females and juvenile eels previously treated with E2, respectively. Expression of aromatase was found only in a single specimen in the control group. In contrast, no difference was found among sexes in the aromatase expression in the brain. Treatment with aromatase inhibitor (AI) of juvenile eel resulted in the total loss of aromatase expression in the gonads and brains. The results of this work reveal...Continue Reading

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Citations

Apr 1, 2005·Fish Physiology and Biochemistry·Francesc Piferrer, Mercedes Blázquez
Jan 22, 2013·Fish Physiology and Biochemistry·Benjamin GeffroyAgnès Bardonnet
Apr 12, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jin Young ChoiSe Jae Kim
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Feb 2, 2010·Frontiers in Neuroendocrinology·Nicolas DiotelOlivier Kah
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Apr 23, 2014·Reproduction in Domestic Animals = Zuchthygiene·D S PeñarandaJ F Asturiano
Jul 29, 2006·General and Comparative Endocrinology·B Scott Nunez, Scott L Applebaum
Jul 11, 2006·Frontiers in Neuroendocrinology·Paul M ForlanoAndrew H Bass
Jul 8, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Elisabeth PellegriniOlivier Kah
May 27, 2014·General and Comparative Endocrinology·Yang ZhangWeimin Zhang
Jun 19, 2013·General and Comparative Endocrinology·Juan I FernandinoGustavo M Somoza
Oct 26, 2012·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·B GeffroyA Bardonnet
Aug 30, 2007·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Makiko KarubeReynaldo Patiño
Jan 26, 2020·General and Comparative Endocrinology·Chien-Ju LinChing-Fong Chang

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