PMID: 2104614Jan 1, 1990Paper

Cloning and DNA sequence of the gene coding for the major sigma factor from Myxococcus xanthus

Journal of Bacteriology
S Inouye

Abstract

The gene for a sigma factor (rpoD) was cloned from Myxococcus xanthus, a soil bacterium which differentiates to form fruiting bodies upon starvation for nutrients. The DNA sequence of the gene was determined, and an open reading frame encoding a polypeptide of 708 amino acid residues (Mr = 80,391) was identified. Except for the amino-terminal sequence consisting of 100 residues, the M. xanthus sigma factor (sigma-80) showed extensive similarity with Escherichia coli sigma-70 as well as Bacillus subtilis sigma-43. In particular, the carboxy-terminal sequence of 242 residues that is known to be required for promoter recognition and core recognition showed 78 and 72% amino acid sequence identity with the E. coli and B. subtilis sigma factors, respectively. The putative RpoD protein was detected at the position of an apparent molecular weight of 86,000 by Western blot (immunoblot) analysis by using antiserum against B. subtilis sigma-43, which agreed well with the position of a vegetative sigma factor of M. xanthus previously identified by Rudd and Zusman (K. Rudd and D. R. Zusman, J. Bacteriol. 151:89-105, 1982).

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Citations

Sep 12, 1998·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·T Ueki, S Inouye
May 5, 2004·The Journal of Biological Chemistry·José Juan López-RubioMontserrat Elías-Arnanz
May 1, 1992·Journal of Molecular Evolution·P Szafrański
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·I M Keseler, D Kaiser
Jun 23, 2006·Applied Microbiology and Biotechnology·Toshiyuki Ueki, Sumiko Inouye
Dec 3, 2005·Journal of Bacteriology·Toshiyuki UekiSumiko Inouye
Jun 1, 1993·Journal of Bacteriology·D Apelian, S Inouye
May 2, 1991·Biochimica Et Biophysica Acta·K Tanaka, H Takahashi
Mar 1, 1991·Journal of Bacteriology·S FujitaniS Inouye
Dec 1, 1995·Journal of Bacteriology·W A HanlonS Inouye
Dec 1, 1990·Microbiological Reviews·L J Shimkets
Oct 1, 1991·Current Opinion in Genetics & Development·H B Kaplan

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