Cloning and expression analysis of a novel mouse gene with sequence similarity to the Drosophila fat facets gene

Mechanisms of Development
S A WoodJ S Mattick

Abstract

The Drosophila fat facets (faf) gene is a ubiquitin-specific protease necessary for the normal development of the eye and of the syncytial stage embryo in the fly. Using a gene trap approach in embryonic stem cells we have isolated a murine gene with extensive sequence similarity to the Drosophila faf gene and called it Fam (fat facets in mouse). The putative mouse protein shows colinearity and a high degree of sequence identity to the Drosophila protein over almost its entire length of 2554 amino acids. The two enzymatic sites characteristic of ubiquitin-specific proteases are very highly conserved between mice and Drosophila and this conservation extends to yeast. Fam is expressed in a complex pattern during postimplantation development. In situ hybridisation detected Fam transcripts in the rapidly expanding cell populations of gastrulating and neurulating embryos, in post-mitotic cells of the CNS as well as in the apoptotic regions between the digits, indicating that it is not associated with a single developmental or cellular event. The strong sequence similarity to faf and the developmentally regulated expression pattern suggest that Fam and the ubiquitin pathway may play a role in determining cell fate in mammals, as has ...Continue Reading

References

Jan 10, 1991·Nature·M GlotzerM W Kirschner
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Apr 1, 1995·Current Opinion in Cell Biology·M Hochstrasser
May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·I WefesD Finley
Jan 1, 1993·Methods in Enzymology·D G Wilkinson, M A Nieto
Oct 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·A M Wunsch, A L Haas

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Citations

Jan 10, 2002·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Janice A Fischer, Erin Overstreet
Dec 22, 1999·Biochemical and Biophysical Research Communications·C H Chung, S H Baek
Dec 12, 2003·Experimental Eye Research·Fu Shang, Allen Taylor
Dec 15, 2000·Progress in Neurobiology·M F Mehler, S Gokhan
Jun 10, 2011·European Journal of Human Genetics : EJHG·Rosario Michael PiroFerdinando Di Cunto
Jan 30, 2009·Molecular Biology of the Cell·Lachlan A JollyStephen A Wood
May 6, 2010·PloS One·Veronika SanderEdward M De Robertis
Oct 29, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Takeshi SatoYoshihiro Hayashi
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·T S TanakaM S Ko
Sep 10, 2014·The Journal of Experimental Medicine·Edwina NaikVishva M Dixit
Jan 5, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S TayaK Kaibuchi
May 22, 2013·Proceedings of the National Academy of Sciences of the United States of America·Yoon ParkYun-Cai Liu
Jun 28, 2005·The European Journal of Neuroscience·J XuA P Arnold
Jul 19, 2005·Brain Research. Molecular Brain Research·Jun Xu
Feb 13, 2015·Cellular and Molecular Life Sciences : CMLS·Mariyam MurtazaStephen A Wood
Dec 4, 2001·Mechanisms of Development·M PantaleonS A Wood
Mar 13, 2014·American Journal of Human Genetics·Claire C HomanLachlan A Jolly
Aug 29, 1998·The Journal of Cell Biology·S TayaK Kaibuchi
Apr 1, 2017·Nature Communications·Xin LiLei Shi
Feb 24, 2018·Frontiers in Cellular Neuroscience·Marta KochBassem A Hassan
Dec 22, 2009·Nature·Martin SchwickartVishva M Dixit
Feb 18, 2020·Irish Journal of Medical Science·Mei-Yuan ChenMing Ma
Jun 18, 2017·The Journal of Biological Chemistry·Qian WangWenshu Ge
Mar 28, 2019·Proceedings of the National Academy of Sciences of the United States of America·Prajwal PaudelYufeng Tong
Jan 4, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Xiaoli HuXueqiong Zhu
Mar 27, 2021·Nature Communications·Trisha A Macrae, Miguel Ramalho-Santos

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