Cloning and expression analysis of CaPIP1-1 gene in pepper (Capsicum annuum L.)

Gene
Yan-Xu YinZhen-Hui Gong

Abstract

Plant aquaporins are responsible for water transmembrane transport, which play an important role on abiotic and biotic stresses. A novel plasma membrane intrinsic protein of CaPIP1-1 was isolated from the pepper P70 according to transcriptome databases of Phytophthora capsici inoculation and chilling stress library. CaPIP1-1, which is 1155 bp in length with an open reading frame of 861 bp, encoded 286 amino acids. Three introns, exhibited CT/AC splice junctions, were observed in CaPIP1-1. The numbers and location of introns in CaPIP1-1 were the same as observed in tomato and potato. CaPIP1-1 was abundantly expressed in pepper fruit. Increased transcription levels of CaPIP1-1 were found in the different stresses, including chilling stress, salt stress, mannitol stress, salicylic acid, ABA treatment and Phytophthora capsici infection. The expression of CaPIP1-1 was downregulated by 50 μM HgCl2 and 100 μM fluridone. The pepper plants silenced CaPIP1-1 in cv. Qiemen showed growth inhibition and decreased tolerance to salt and mannitol stresses using detached leaf method.

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Citations

Nov 6, 2018·Current Protein & Peptide Science·João P Bezerra-NetoAna M Benko-Iseppon
May 1, 2019·Molecular Plant-microbe Interactions : MPMI·Zhiqin LiuShuilin He
Mar 17, 2020·Frontiers in Plant Science·Huai-Xia ZhangZhen-Hui Gong
Jan 16, 2020·International Journal of Molecular Sciences·Huai-Xia ZhangZhen-Hui Gong
May 11, 2017·Frontiers in Plant Science·Li WangJunlian Zhang
Jun 7, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·U Lakshmi SahityaP Suneetha

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