Cloning and expression analysis of SKn-type dehydrin gene from bean in response to heavy metals.

Molecular Biotechnology
Yuxiu ZhangTuanyao Chai

Abstract

A heavy metal responsive gene PvSR3 (GenBank accession number U54703) encoding an acid dehydrin was isolated from a mercuric chloride-treated bean (Phaseolus vulgaris L.) leaf cDNA library by differential screening using cDNAs derived from treated and untreated plants. The PvSR3 cDNA is 981-bp long and has a 606-bp open-reading frame with a 202-residue-deduced amino acid sequence. The PvSR3 sequence contains two conserved repeats of the characteristic lysine-rich K segment (EKKGIMDKIKEKLPG) preceded by an 8-serine residue stretch, whereas the Y segment (DEYGNP) conserved motif is absent. The deduced protein has a calculated molecular weight of 23 kDa and an isoelectric point of 5.2. Sequence similarity and comparative analysis showed that PvSR3 shares 70 and 73% similarity with the dehydrin of poplar and pepper, respectively. Southern hybridizations indicated that PvSR3 was a low copy-number gene. Northern blot analysis revealed that PvSR3 mRNA was weakly detected in seedling leaves. However, the gene expression was strongly stimulated by heavy metals, such as mercury, cadmium, arsenic, and copper, whereas virus infection and salt had little effect on it. In contrast, PvSR3 was not responsive to drought or abscisic acid (ABA), ...Continue Reading

Citations

May 5, 2007·Die Naturwissenschaften·Alan Tunnacliffe, Michael J Wise
Jan 26, 2008·Molecular Biotechnology·Jin XuTuan Yao Chai
May 15, 2008·Heredity·S C González-MartínezD B Neale
Feb 24, 2016·Frontiers in Plant Science·Samiksha SinghSheo M Prasad
Jun 30, 2011·Protein Expression and Purification·Francisco CamposAlejandra A Covarrubias
Nov 18, 2014·Frontiers in Plant Science·Steffen P Graether, Kelly F Boddington
Nov 6, 2018·International Journal of Molecular Sciences·Zhengyang YuLinsheng Zhang
Mar 2, 2018·Frontiers in Plant Science·Tanmoy HalderSudipta Ray
Dec 21, 2021·Plant Molecular Biology·Ľubica LiptákováLadislav Tamás

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