Cloning and Expression Analysis of ZmERD3 Gene From Zea mays

Iranian Journal of Biotechnology
Xiaoqing SongYinghui Liu

Abstract

Stresses (such as drought, salt, viruses, and others) seriously affect plant productivity. To cope with these threats, plants express a large number of genes, including several members of ERD (early responsive to dehydration) genes to synthesize and assemble adaptive molecules. But, the function of ERD3 gene hasn't been known so far. The purpose of the present study was to clone the stress-resistance gene: ZmERD3, and to analyze its expression pattern in the maize plant organs at different stages and under various stress treatments. MaizeGDB database search together with the bioinformatics analysis led to the identification of ZmERD3 gene in Zea mays. The cDNA sequence and promoter of ZmERD3 gene were obtained through PCR. Bioinformatics analysis was performed through online tools. The tissue-specific expression profile of the ZmERD3 gene in maize plant was carried out using the quantitative real time PCR (qRT-PCR) technique and its expression pattern in response to stress treatments (such as PEG, NaCl, ABA, and low temperature) was also analyzed through qRT-PCR method. Based on the homology alignment with AtERD3 (XP_002867953) in MaizeGDB (http://www. maizegdb.org/), the cDNA sequence and promoter region of the ZmERD3 gene w...Continue Reading

References

Jul 18, 2000·The Journal of Biological Chemistry·A NyyssolaT Reinikainen
Mar 29, 2003·Trends in Plant Science·Shahid MansoorJohn Stanley
Jun 27, 2003·Trends in Biochemical Sciences·Heidi L SchubertXiaodong Cheng
Jan 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Rungaroon WaditeeTeruhiro Takabe
Mar 21, 2006·Biochemical Society Transactions·W A M Loenen
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May 10, 2011·Science·David B LobellJustin Costa-Roberts
Mar 13, 2014·Genetics and Molecular Research : GMR·H T WangH Xing

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Citations

Feb 10, 2021·Plants·Muhammad Ahmed WaqasMuhammad Farooq

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Datasets Mentioned

BETA
KU360141

Methods Mentioned

BETA
PCR
transgenic

Software Mentioned

WoLFPSORT
ProtComp
NCBICDS
YLoc
signalP
MODEL
Blast
DNAman
PlantCARE
Clustal X

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