Cloning and expression in Escherichia coli K-12 of the genes for major outer membrane protein OmpA from Shigella dysenteriae, Enterobacter aerogenes, and Serratia marcescens.

Journal of Bacteriology
S T ColeU Henning

Abstract

The outer membranes of many gram-negative bacteria contain a major heat-modifiable protein which shows serological cross-reactivity with the OmpA protein of Escherichia coli K-12. Using the cloned gene for the E. coli K12 protein as a DNA-DNA hybridization probe, we were able to identify the corresponding genes from Shigella dysenteriae. Enterobacter aerogenes, and Serratia marcescens. These were cloned in a phage lambda vector, and their expression in E. coli K-12 was studied. All three OmpA proteins were fully produced and correctly exported to the outer membrane. In several cases, complete or partial restoration of known function of the E. coli K-12 protein was observed.

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Citations

Feb 1, 1988·Klinische Wochenschrift·S Pistor, G Hobom
Jan 1, 1985·CRC Critical Reviews in Biochemistry·R Benz
Jan 1, 1983·Molecular & General Genetics : MGG·S T Cole
Aug 15, 1983·European Journal of Biochemistry·R Freudl, S T Cole
May 10, 2000·Journal of Microbiological Methods·G Mardones, A Venegas
Jun 1, 2002·Journal of Biotechnology·Patrik SamuelsonStefan Ståhl
Mar 1, 1985·Microbiological Reviews·H Nikaido, M Vaara

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