PMID: 2493454Mar 1, 1989Paper

Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation

Journal of Bacteriology
F J Mondello

Abstract

Pseudomonas strain LB400 is able to degrade an unusually wide variety of polychlorinated biphenyls (PCBs). A genomic library of LB400 was constructed by using the broad-host-range cosmid pMMB34 and introduced into Escherichia coli. Approximately 1,600 recombinant clones were tested, and 5 that expressed 2,3-dihydroxybiphenyl dioxygenase activity were found. This enzyme is encoded by the bphC gene of the 2,3-dioxygenase pathway for PCB-biphenyl metabolism. Two recombinant plasmids encoding the ability to transform PCBs to chlorobenzoic acids were identified, and one of these, pGEM410, was chosen for further study. The PCB-degrading genes (bphA, -B, -C, and -D) were localized by subcloning experiments to a 12.4-kilobase region of pGEM410. The ability of recombinant strains to degrade PCBs was compared with that of the wild type. In resting-cell assays, PCB degradation by E. coli strain FM4560 (containing a pGEM410 derivative) approached that of LB400 and was significantly greater than degradation by the original recombinant strain. High levels of PCB metabolism by FM4560 did not depend on the growth of the organism on biphenyl, as it did for PCB metabolism by LB400. When cells were grown with succinate as the carbon source, PCB d...Continue Reading

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Citations

Jan 1, 1993·Applied Biochemistry and Biotechnology·D C Cameron, I T Tong
Oct 15, 1993·FEMS Microbiology Letters·D N DowlingD F Dwyer
Aug 15, 1997·FEMS Microbiology Letters·F ArtiguenaveC Danglot
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Feb 9, 2000·Folia Microbiologica·N G Rojas-AvelizapaG Fernández-Villagómez
Dec 27, 2002·The Journal of General and Applied Microbiology·Michihisa MaedaToshiaki Kudo
Oct 1, 2019·Microbial Biotechnology·Panu PimviriyakulPimchai Chaiyen
Jan 1, 1989·Critical Reviews in Biotechnology·F S Sariaslani
Apr 20, 2001·The Journal of Biological Chemistry·H SuenagaK Furukawa
Dec 1, 1994·Biodegradation·V BrennerD D Focht
Dec 17, 2002·The Journal of General and Applied Microbiology·Kensuke Furukawa
May 2, 1994·Annals of the New York Academy of Sciences·A C LaytonG S Sayler
Dec 23, 2004·Applied Microbiology and Biotechnology·Dietmar H Pieper
Jun 12, 2002·Journal of Bacteriology·Hikaru SuenagaKensuke Furukawa
Aug 5, 2004·Journal of Bacteriology·Kensuke FurukawaMasatoshi Goto
Jul 8, 2005·Applied and Environmental Microbiology·George W PayneEshwar Mahenthiralingam
Feb 18, 2006·Journal of Bacteriology·Muriel GaillardJan Roelof van der Meer

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