Cloning and expression of a β-mannanase gene from Bacillus sp. MK-2 and its directed evolution by random mutagenesis

Enzyme and Microbial Technology
Wen ZhangRuifu Zhang

Abstract

A β-mannanase gene was cloned from Bacillus sp. MK-2 and expressed in Bacillus subtilis WB800. The ORF of the β-mannanase gene was 1104 bp in length, encoding 367 aa. The deduced amino acid sequence shared high sequence identity with the β-mannanase from Bacillus subtilis, and belongs to glycosyl hydrolase family 26. The purified recombinant enzyme had a specific activity of 2802 U/mg and displayed optimum activity at pH 6.0 and 55 °C. To obtain an enzyme with high specific activity and facilitate its industrial applications, molecular engineering of Bman26 was undertaken using random mutagenesis in Bacillus subtilis WB800. Three positive mutants with substantially improved specific activities were selected and studied. The best performing mutant was K291E, for which the single amino acid substitution led to a 3.5-fold increase in kcat/Km. Mutants Q112R and L211I also exhibited an apparently increased kcat/Km towards konjac glucomannan, approximately 200% and 80% improvement, respectively. Structural-functional analysis indicated that a slight conformational change could dramatically affect certain enzyme characteristics. In addition, three amino acid sites (Gly88-Leu212-Lys288) in Bman26 were found to have close relationships ...Continue Reading

Citations

Aug 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lucas Ferreira RibeiroMaría-Eugenia Guazzaroni
Oct 10, 2020·Critical Reviews in Biotechnology·Gaurav Singh Kaira, Mukesh Kapoor
Jan 2, 2021·Frontiers in Bioengineering and Biotechnology·Aneesa Dawood, Kesen Ma
Dec 6, 2020·International Journal of Biological Macromolecules·Zhemin LiuHaijin Mou
Nov 27, 2021·Preparative Biochemistry & Biotechnology·Yangcun SunJingping Ge

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