Cloning and expression of a 5'-iodothyronine deiodinase from the liver of Fundulus heteroclitus

Endocrinology
C ValverdeD L Germain

Abstract

Recent molecular cloning studies in mammals and amphibians have demonstrated that the types I, II, and III deiodinases constitute a family of selenoproteins of critical importance in metabolizing T4 to active (i.e. T3) and inactive (i.e. rT3) metabolites. In several tissues of teleost fish, various deiodinase processes have been described, but the structural and functional characteristics of these enzymes and their relationship to the deiodinases present in higher vertebrates remains uncertain. Using a complementary DNA library derived from the liver of the teleost Fundulus heteroclitus, we have identified a complementary DNA that codes for a deiodinase with functional characteristics virtually identical to those of the mammalian and amphibian type II deiodinase. Sequence analysis demonstrates a high degree of homology at both the nucleotide and predicted amino acid levels between the Fundulus clone and these previously characterized type II enzymes, including the presence of an in-frame TGA codon that codes for selenocysteine. These findings demonstrate that the deiodinase family of selenoproteins has been highly conserved during vertebrate evolution and underscores their importance in the regulation of thyroid hormone action.

Citations

Dec 31, 2003·General and Comparative Endocrinology·Carlota García-GAurea Orozco
Jun 1, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Ana Maria CoimbraVeerle M Darras
May 24, 2014·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Lucia A SealeAndre P Seale
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Dec 6, 2003·Endocrinology·Caroline A ShepherdleyGeorge G J M Kuiper
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Jul 18, 1997·The Journal of Biological Chemistry·S PalludD L St Germain
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Dec 11, 2017·Best Practice & Research. Clinical Endocrinology & Metabolism·Keisuke Ikegami, Takashi Yoshimura

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