PMID: 7544779Sep 1, 1995Paper

Cloning and expression of a cDNA encoding bovine retinal pigment epithelial 11-cis retinol dehydrogenase

Investigative Ophthalmology & Visual Science
C A DriessenJ J Janssen

Abstract

Identification of a 32-kd protein in the bovine retinal pigment epithelium. A bovine retinal pigment epithelium cDNA library was constructed in the bacteriophage lambda ZAP Express. A monoclonal antibody, designated 21-C3/AV, was used to isolate the cDNA encoding the 21-C3/AV antigen. A positive full-length clone, designated 21-C3RDH/CD, was sequenced. Northern blot analysis was used to determine the length of the mRNA and the tissue expression pattern. The entire open reading frame of clone 21-C3RDH/CD was used to isolate a recombinant baculovirus clone and expressed in Spodoptera frugiperda insect cells. Enzymatic activity toward 11-cis retinaldehyde was investigated. The complete nucleotide sequence of 21-C3RDH/CD was obtained. The deduced amino acid sequence reveals homology with short-chain alcohol dehydrogenases. Northern blot analysis detected a 1.2-kb transcript. Although the monoclonal antibody used to isolate 21-C3RDH/CD also reacts with other ocular and nonocular tissues, the authors were unable to demonstrate any reactivity with RNA samples isolated from different (non)ocular tissues. Recombinant baculovirus-infected insect cells synthesized the 21-C3/AV antigen. This protein showed 11-cis retinol dehydrogenase acti...Continue Reading

Citations

Jan 13, 1998·Gene·C A DriessenJ J Janssen
Aug 2, 2003·Progress in Retinal and Eye Research·Debra A Thompson, Andreas Gal
Oct 16, 1999·Biochimica Et Biophysica Acta·J L Napoli
Sep 13, 2002·The Journal of Biological Chemistry·Françoise HaeseleerKrzysztof Palczewski
May 17, 2007·The Journal of Biological Chemistry·Minghao JinGabriel H Travis
Sep 2, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Takehiro G KusakabeMotoyuki Tsuda
Sep 14, 2006·Annual Review of Pharmacology and Toxicology·Gabriel H TravisKrzysztof Palczewski
Jun 17, 2014·Progress in Retinal and Eye Research·Evelina BertolottiValeria Marigo
Apr 28, 2006·Acta Ophthalmologica Scandinavica·Takaaki HayashiKenji Kitahara
Nov 13, 2003·Vision Research·Carola DriessenJacques Janssen
Feb 8, 2003·American Journal of Respiratory and Critical Care Medicine·Satoshi SuzukiZygmund S Krozowski

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