Cloning and expression of a ferritin subunit for Galleria mellonella

Archives of Insect Biochemistry and Physiology
B S KimH R Kim

Abstract

Ferritin was purified from iron-fed Galleria mellonella hemolymph by ultra centrifugation and FPLC (Superose 6). SDS-PAGE revealed three bands of 26, 30, and 32 kDa. The ferritin 26 kDa subunit cDNA was obtained from RT-PCR using primer designed from N-terminal sequence analysis. 5'-RACE was used to obtain the complete protein coding sequence. The sequence encodes a 211 amino acid polypeptide including a 20 amino acid leader peptide. An IRE (iron-responsive element) sequence with a predicted stem-loop structure was present in the 5'-UTR of ferritin mRNA. Sequence alignment has a sequence identity with Calpodes ethlius (S)(74%), Drosophila melanogaster (50%), and Aedes aegypti (39%). Northern blot analysis indicated that there were 1.5- and 1.75-fold increases in the expression of ferritin mRNA after iron-fed fat body and midgut, respectively. Also, we confirmed that the ferritin mRNA is not expressed in adult ovary and testis. Arch.

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Citations

Dec 1, 2001·Annual Review of Entomology·Helen NicholJoy J Winzerling
Mar 10, 2009·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bo Yeon KimByung Rae Jin
Nov 14, 2006·Insect Biochemistry and Molecular Biology·Patricia M Strickler-DinglasanSerap Aksoy
Apr 22, 2004·Archives of Insect Biochemistry and Physiology·Hong Ja KimSook Jae Seo
Sep 17, 2002·Archives of Insect Biochemistry and Physiology·Beom Su KimHak R Kim
Sep 21, 2018·PLoS Pathogens·Cristina Colomer-WinterJosé A Lemos
Jun 2, 2005·Insect Molecular Biology·D Q-D Pham, C A Chavez
Oct 20, 2005·Archives of Insect Biochemistry and Physiology·Lihong QiuJ Marshall Clark
Mar 30, 2018·European Biophysics Journal : EBJ·Eliane WajnbergMaria de Lourdes Barriviera
Mar 17, 2010·Biochimica Et Biophysica Acta·Daphne Q D Pham, Joy J Winzerling

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