PMID: 9555100Jun 20, 1998Paper

Cloning and expression of a group IV cytosolic Ca2+-dependent phospholipase A2 from rat pancreatic islets. Comparison of the expressed activity with that of an islet group VI cytosolic Ca2+-independent phospholipase A2

Biochimica Et Biophysica Acta
Z MaJohn Turk

Abstract

Stimulation of pancreatic islets with glucose induces phospholipid hydrolysis and accumulation of nonesterified arachidonic acid, which may play signaling or effector roles in insulin secretion. Of enzymes that catalyze phospholipid hydrolysis, islet beta-cells express low molecular weight secretory phospholipases A2 (PLA2) and a Group VI, Ca2+-independent PLA2 (iPLA2). Previous studies indicate that islets also express a protein recognized by antibodies against a Group IV, cytosolic, Ca2+-dependent PLA2 (cPLA2). To further examine the possible expression of cPLA2 by islets, we screened a rat islet cDNA library with a probe that recognizes cPLA2 sequence, and isolated a full-length cPLA2 cDNA. The rat islet cPLA2-deduced amino acid sequence is 96% identical to those of human and mouse cPLA2. Transfection of COS-7 cells with cPLA2 cDNA in an expression vector induced expression of Ca2+-dependent PLA2 activity and of a protein recognized by anti-cPLA2 antibody. Comparison of recombinant islet cPLA2 and iPLA2 activities expressed in transfected COS-7 cells indicated that iPLA2 but not cPLA2 is stimulated by ATP. Both activities are similarly sensitive to inhibition by arachidonyltrifluoromethyl ketone, but iPLA2 is more effectivel...Continue Reading

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Citations

May 29, 2007·Biochemical Pharmacology·Brian S Cummings
Jan 30, 2004·Diabetes·Peter M JonesShanta J Persaud
Dec 2, 2004·Canadian Journal of Physiology and Pharmacology·John Turk, Sasanka Ramanadham
Sep 1, 2007·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Zuzana BacováVladimir Strbák
May 30, 2015·Journal of Lipid Research·Sasanka RamanadhamXiaoyong Lei
Nov 19, 2004·The Journal of Biological Chemistry·Nagadhara DronadulaGadiparthi N Rao
Jan 24, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Martin KöhlerPer-Olof Berggren
Mar 20, 2003·American Journal of Physiology. Endocrinology and Metabolism·Kirstine JuhlJesper Gromada
Nov 25, 2003·The Journal of Pharmacology and Experimental Therapeutics·Brian S CummingsRick G Schnellmann
Aug 22, 2006·Gynecologic Oncology·Michal GorovetzReuven Reich

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