Cloning and expression of a novel NADP(H)-dependent daidzein reductase, an enzyme involved in the metabolism of daidzein, from equol-producing Lactococcus strain 20-92.

Applied and Environmental Microbiology
Yoshikazu ShimadaHaretsugu Hishigaki

Abstract

Equol is a metabolite produced from daidzein by enteric microflora, and it has attracted a great deal of attention because of its protective or ameliorative ability against several sex hormone-dependent diseases (e.g., menopausal disorder and lower bone density), which is more potent than that of other isoflavonoids. We purified a novel NADP(H)-dependent daidzein reductase (L-DZNR) from Lactococcus strain 20-92 (Lactococcus 20-92; S. Uchiyama, T. Ueno, and T. Suzuki, international patent WO2005/000042) that is involved in the metabolism of soy isoflavones and equol production and converts daidzein to dihydrodaidzein. Partial amino acid sequences were determined from purified L-DZNR, and the gene encoding L-DZNR was cloned. The nucleotide sequence of this gene consists of an open reading frame of 1,935 nucleotides, and the deduced amino acid sequence consists of 644 amino acids. L-DZNR contains two cofactor binding motifs and an 4Fe-4S cluster. It was further suggested that L-DZNR was an NAD(H)/NADP(H):flavin oxidoreductase belonging to the old yellow enzyme (OYE) family. Recombinant histidine-tagged L-DZNR was expressed in Escherichia coli. The recombinant protein converted daidzein to (S)-dihydrodaidzein with enantioselectivit...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M A FrohmanG R Martin
Aug 7, 1969·Biochemical and Biophysical Research Communications·K HayashiT Suzuki
Nov 1, 1981·European Journal of Biochemistry·R Untucht-GrauR L Krauth-Siegel
Jan 1, 1982·European Journal of Biochemistry·R L Krauth-SiegelR Untucht-Grau
Aug 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·G E JoannouC Nelson
Nov 4, 2000·The Journal of Biological Chemistry·F RohdichA Bacher
Jul 11, 2002·The Lancet Oncology·Herman Adlercreutz
Dec 7, 2002·The Journal of Nutrition·Kenneth D R SetchellEva Lydeking-Olsen
Feb 25, 2003·Bioscience, Biotechnology, and Biochemistry·Michihiko KataokaSakayu Shimizu
Mar 28, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E BoweyI Rowland
Dec 11, 2003·Journal of Agricultural and Food Chemistry·Drazen KostelacKarlis Briviba
Oct 19, 2006·Journal of Bioscience and Bioengineering·Kimiko MinamidaKozo Asano
Mar 11, 2008·FEMS Microbiology Letters·Zhuo-Teng YuWei-Yun Zhu
May 3, 2008·International Journal of Systematic and Evolutionary Microbiology·Toshinari MaruoYoshimi Benno
May 3, 2008·International Journal of Systematic and Evolutionary Microbiology·Kimiko MinamidaKozo Asano
Oct 8, 2008·Bioscience, Biotechnology, and Biochemistry·Shin-ichiro Yokoyama, Tohru Suzuki

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Citations

May 15, 2012·Applied and Environmental Microbiology·Yoshikazu ShimadaHaretsugu Hishigaki
Dec 20, 2011·Applied and Environmental Microbiology·Hirokazu TsujiHideyuki Akaza
Jan 31, 2012·Journal of Molecular Microbiology and Biotechnology·Yoshikazu ShimadaHaretsugu Hishigaki
Apr 2, 2008·BMC Biotechnology·Marine GoffinetJean-Charles Faye
May 25, 2013·Journal of Clinical Biochemistry and Nutrition·Mariko Uehara
Aug 21, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Henry J Haiser, Peter J Turnbaugh
Jun 9, 2016·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Jan F Stevens, Claudia S Maier
Mar 11, 2016·Gut Microbes·Annett Braune, Michael Blaut
Apr 8, 2015·Critical Reviews in Food Science and Nutrition·J M LandeteR Muñoz
May 18, 2016·Applied Microbiology and Biotechnology·Michihiko KataokaMasaru Tanokura
Aug 11, 2016·Bioscience of Microbiota, Food and Health·Yuika KawadaTohru Suzuki
Mar 6, 2018·Bioscience, Biotechnology, and Biochemistry·Kaeko MurotaMariko Uehara
Nov 1, 2017·European Journal of Nutrition·Colette ShorttJean-Michel Antoine
Jul 23, 2013·International Journal of Food Sciences and Nutrition·Yasuhiro AbiruShigeto Uchiyama
Mar 21, 2020·Molecular Nutrition & Food Research·Adrián Cortés-MartínJuan Carlos Espín
Nov 8, 2017·BioMed Research International·José María LandeteJuan L Arqués
Sep 6, 2018·FEMS Microbiology Letters·Pyung-Gang LeeByung-Gee Kim
Mar 22, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ludmila KřížováTomáš Kašparovský
Feb 3, 2021·Antioxidants·Małgorzata MakarewiczAleksandra Duda-Chodak
Jul 13, 2021·Critical Reviews in Food Science and Nutrition·Saied A AboushanabElena G Kovaleva
Jun 27, 2021·FEMS Microbiology Letters·Lucía VázquezBaltasar Mayo
Oct 2, 2021·Scientific Reports·Tomoko FujitaniKouji H Harada

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