Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase

European Journal of Biochemistry
Christiane StuhlfelderH Warzecha

Abstract

Jasmonic acid and its methyl ester are ubiquitous plant signalling compounds necessary for the regulation of growth and development, as well as for the response of plants to environmental stress factors. To date, it is not clear whether methyl jasmonate itself acts as a signal or if its conversion to jasmonic acid is mandatory prior to the induction of a defense response. We have cloned a cDNA, encoding a methyl jasmonate-cleaving enzyme, from tomato cell suspension cultures. Sequence analysis revealed significant similarity to plant esterases and to (S)-hydroxynitrile lyases with an alpha/beta-hydrolase fold structure. The coding sequence was heterologously expressed in Escherichia coli and purified in a catalytically active form. Transcript levels, as well as enzymatic activity, were determined in different tomato tissues. High transcript levels and enzyme activities were found in roots and flowers, while the mRNA level and activity were low in stems and leaves. Moreover, when tested in methyl jasmonate- and elicitor-treated cell suspension cultures, transcript levels were found to decrease, indicating that this particular enzyme might be a regulator of jasmonate signalling.

References

Mar 4, 1998·Plant Molecular Biology·R K IbrahimB Bantignies
Aug 15, 1998·Archives of Biochemistry and Biophysics·J HughesM A Hughes
Jul 15, 1999·Structure·P HeikinheimoD L Ollis
Oct 25, 2000·Trends in Plant Science·E Pichersky, D R Gang
Feb 22, 2001·Journal of Experimental Botany·F Schaller
Apr 5, 2001·Proceedings of the National Academy of Sciences of the United States of America·H S SeoY D Choi
Oct 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·A StintziE E Farmer
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E E Farmer, C A Ryan
Mar 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H GundlachM H Zenk
Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·P E StaswickS H Howell
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S H DoaresC A Ryan
Oct 25, 2001·Proceedings of the National Academy of Sciences of the United States of America·G A Howe
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Lei LiGregg A Howe
May 7, 2002·Trends in Plant Science·Hans Weber
May 29, 2002·Phytochemistry·Christiane StuhlfelderMartin J Mueller
Jun 24, 2003·Trends in Plant Science·Johannes W Stratmann
Dec 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dhirendra Kumar, Daniel F Klessig
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Robert A. Creelman, John E. Mullet
Mar 1, 1985·Plant Molecular Biology·S O Rogers, A J Bendich

❮ Previous
Next ❯

Citations

Feb 14, 2007·Plant Cell Reports·Choonkyun JungJong-Joo Cheong
Apr 13, 2007·Molecular Genetics and Genomics : MGG·Takashi HikageKen-Ichi Tsutsumi
Oct 28, 2010·Molecular Genetics and Genomics : MGG·Takashi HikageKen-Ichi Tsutsumi
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Chang-jun HouCai-hong Shen
Apr 27, 2013·Applied Biochemistry and Biotechnology·Limin YangChangjun Hou
Jun 9, 2005·Current Opinion in Plant Biology·Anthony L Schilmiller, Gregg A Howe
Jan 16, 2007·The Plant Cell·Marina VarbanovaEran Pichersky
May 3, 2007·The Plant Cell·Nancy A Eckardt
Aug 5, 2005·Annual Review of Phytopathology·Jyoti Shah
Jan 26, 2005·Proceedings of the National Academy of Sciences of the United States of America·Farhad ForouharLiang Tong
Jul 12, 2007·Plant & Cell Physiology·Nausicaä LannooEls J M Van Damme
May 7, 2009·Plant & Cell Physiology·Gianni VandenborreEls J M Van Damme
Jan 27, 2011·Plant & Cell Physiology·Chizuru SatoHideyuki Matsuura
Mar 30, 2010·Plant Physiology and Biochemistry : PPB·Walter P SuzaArgelia Lorence
Dec 25, 2015·Molecular Genetics and Genomics : MGG·Takashi HikageKen-Ichi Tsutsumi
Apr 6, 2005·The Plant Journal : for Cell and Molecular Biology·Srinivasa Rao UppalapatiCarol L Bender
Jun 7, 2006·Current Opinion in Plant Biology·Murray Grant, Chris Lamb
Dec 22, 2005·Journal of Plant Physiology·Claus WasternackOtto Miersch
May 23, 2015·The Plant Journal : for Cell and Molecular Biology·Taiji NomuraYasuo Kato
Feb 8, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yeon Jong KooYang Do Choi
Dec 11, 2007·Journal of Experimental Botany·Wioletta E PluskotaIan T Baldwin
Feb 1, 2014·Functional Plant Biology : FPB·Simone FindlingSusanne Berger
Apr 19, 2019·Journal of Agricultural and Food Chemistry·Xiangmei CaoBo Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosynthetic Transformations

Biosyntheic transformtions are multi-step, enzyme-catalyzed processes where substrates are converted into more complex products in living organisms. Simple compounds are modified, converted into other compounds, or joined together to form macromolecules. Discover the latest research on biosynthetic transformations here.