Cloning and expression of ADAM-related metalloproteases in equine laminitis.

Veterinary Immunology and Immunopathology
Michael J CoyneDominique Alfandari

Abstract

Equine laminitis is a debilitating disease affecting the digital laminae that suspend the distal phalanx within the hoof. While the clinical progression of the disease has been well documented, the molecular events associated with its pathogenesis remain largely unknown. Using real time quantitative PCR (RT-qPCR), we have investigated the expression of genes coding for proteins containing a Disintegrin and Metalloprotease domain (ADAM), as well as genes encoding the natural inhibitors of these enzymes (tissue inhibitor of metalloprotease; TIMP) in horses with naturally-acquired (acute, chronic and aggravated chronic clinical cases) or experimentally-induced (black walnut extract (BWE) and starch gruel models) laminitis. Changes in expression of these enzymes and regulators may underlie the pathologic remodeling of lamellar tissue in laminitis. Genes encoding ADAMs involved in inflammation (ADAM-10 and ADAM-17), as well as those implicated in arthritis (ADAMTS-1, ADAMTS-4 and ADAMTS-5) were cloned, and the sequences used to generate specific oligonucleotide primers for the RT-qPCR experiments. Our results show that genes encoding ADAM-10 and ADAM-17 were not induced in most laminitic animals, whereas ADAMTS-4 gene expression was...Continue Reading

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Apr 26, 2014·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Nicola J Menzies-GowJ Elliott
Jan 14, 2012·Veterinary Immunology and Immunopathology·M A de LaatC C Pollitt
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Jan 5, 2021·Frontiers in Veterinary Science·Shuaichen LiNa Zhang

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