PMID: 1784818Sep 1, 1991Paper

Cloning and expression of an amylase gene from Bacillus stearothermophilus

Research in Microbiology
B DiderichsenP L Jørgensen


In the industrial process of liquefying starch to make glucose or high fructose syrups, it is crucial that the amylase used is stable and active at about 105 degrees C at pH 6.5 or preferentially at a lower pH. The amylase from Bacillus licheniformis is well suited for this purpose but it is possible that other amylases might perform even better. Therefore, we cloned and characterized amyS encoding a heat-stable alpha-amylase from Bacillus stearothermophilus. Using a newly developed method for creating exact gene fusions by in vivo recombination, we attempted to increase expression of amyS in Bacillus subtilis. However, only by introducing the amyS gene into B. licheniformis, we obtained significantly better yields.


Jan 15, 1991·FEMS Microbiology Letters·P L JørgensenB Diderichsen
Jul 1, 1985·Journal of Bacteriology·R NakajimaS Aiba
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J R Mielenz


Aug 3, 2004·Biotechnology and Bioengineering·Jeng-Jie WangJason C H Shih
Oct 5, 2018·Microbial Cell Factories·Pedro H LebreDon A Cowan
Nov 1, 1995·Applied and Environmental Microbiology·A B FlemingF G Priest

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