Abstract
In this paper we cloned, sequenced and expressed a novel Entamoeba histolytica alcohol dehydrogenase gene (Ehadh3). Ehadh3 has a predicted 383 amino acids open reading frame, encoding for a 42.3 kDa protein. The deduced amino acid sequence showed 24 to 26% identity to other type III alcohol dehydrogenases found in prokaryotic and lower eukaryotic organisms, but not in mammalia. There are at least two Ehadh3 gene copies in the genome, but only a 1.2 kb transcript was detected. The EhADH3 fusion protein showed a NADP+(-)dependent ADH activity. Ehadh3 may be a good target for the developing of anti-E. histolytica drugs, without producing damage to the human.
References
Sep 1, 1992·Molecular and Biochemical Parasitology·S DescoteauxJ Samuelson
Nov 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A KumarJ Samuelson
May 30, 1989·Gene·J S YounglesonD R Woods
Dec 21, 1989·Gene·P E GoodloveD P Clark
Aug 8, 1989·Biochemistry·M Peretz, Y Burstein
Sep 1, 1987·Molecular & General Genetics : MGG·V M Williamson, C E Paquin
Jan 1, 1986·Biochemical Pharmacology·M Müller
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·R A Young, R W Davis
Apr 1, 1994·Molecular and Biochemical Parasitology·W YangS L Stanley
Sep 2, 1994·Gene·M A SánchezE Orozco
Aug 1, 1994·Thorax·S M NasserJ P Arm
Mar 2, 2012·The Journal of Physical Chemistry. B·John T KingKevin J Kubarych
Citations
Aug 5, 2000·Molecular and Biochemical Parasitology·M Dan, C C Wang
Apr 15, 2009·PLoS Neglected Tropical Diseases·Paul H DavisSamuel L Stanley
Feb 11, 2010·Journal of Biomedicine & Biotechnology·Isabel Wong-BaezaArmando Isibasi
Jun 17, 2000·Infection and Immunity·S GhoshJ Samuelson
Nov 29, 2002·Eukaryotic Cell·Julie E J NixonJohn Samuelson
May 13, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Marek Mentel, William Martin
Jun 13, 2012·Microbiology and Molecular Biology Reviews : MMBR·Miklós MüllerWilliam F Martin
Dec 4, 2012·FEBS Letters·Erika PinedaEmma Saavedra
Nov 9, 2000·Molecular Microbiology·J FieldJ Samuelson
Jun 13, 2002·The Journal of Parasitology·Eva E AvilaRoberto Zazueta-Sandoval
Mar 29, 2001·The Journal of Biological Chemistry·A EspinosaS L Stanley
Nov 5, 2019·Biochimica Et Biophysica Acta. Proteins and Proteomics·Poonam KumariSamudrala Gourinath
Oct 23, 2020·Microorganisms·Constantin KönigIris Bruchhaus
Jul 2, 1999·Antimicrobial Agents and Chemotherapy·J Samuelson
Jun 1, 1997·Journal of Bacteriology·B RosenthalJ Samuelson