Cloning and expression of caprine interferon-gamma

Gene
J C BeyerW P Cheevers

Abstract

Caprine interferon-gamma (IFN-gamma) cDNA was cloned from mitogen stimulated peripheral blood mononuclear cell (PBMC) RNA utilizing the reverse transcription-polymerase chain reaction (RT-PCR). The cDNA open reading frame (ORF) is 498bp, encoding a putative 166 amino acid (aa) protein (19327Da). The predicted aa sequence homology of caprine IFN-gamma and the corresponding ovine, bovine and cervine cytokine is 98.8%, 95.2% and 92.8%, respectively. IFN-gamma cDNA was subcloned and expressed in two different plasmids under the control of either the human cytomegalovirus (CMV) immediate early promoter or the caprine arthritis-encephalitis virus long terminal repeat (CAEV LTR). Recombinant caprine IFN-gamma (rCaIFN-gamma) secreted by transfected COS-7 cells shared at least two antigenic epitopes with recombinant bovine IFN-gamma (rBoIFN-gamma) and exhibited biological activity in the vesicular stomatitis virus (VSV) cytopathic effect reduction assay. In-vivo expression of IFN-gamma cDNA promoted by the CAEV LTR was confirmed by the intramuscular (IM) injection of Balb/C mice with plasmid followed by Western blot analysis of mouse serum against purified rCaIFN-gamma produced in E. coli.

References

Aug 26, 1982·Nature·P W Gray, D V Goeddel
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Oct 1, 1996·Veterinary Immunology and Immunopathology·M L CrossJ F Griffin

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Citations

Jul 24, 2010·Applied Biochemistry and Biotechnology·Gholamreza Nikbakht BrujeniMohammad Kazem Koohi
May 12, 2005·Veterinary Immunology and Immunopathology·Anne K StorsetBerit Djønne
Sep 12, 2000·Developmental and Comparative Immunology·S LawsonP Kaiser
Apr 9, 2001·Veterinary Immunology and Immunopathology·K R SnekvikW P Cheevers
Apr 13, 2007·Annual Review of Nutrition·Catherine PosticJean Girard
Jun 21, 2007·Upsala Journal of Medical Sciences·Torvard C Laurent
Sep 1, 2015·Veterinary Immunology and Immunopathology·Sin Ying ShenKuo Pin Chuang
Jul 21, 2001·Tuberculosis·P R Wood, S L Jones
Nov 9, 2011·Research in Veterinary Science·Mariarosaria MarinaroGiovanni Rezza
Nov 8, 2014·Animal Biotechnology·Sunil Shebannavar, Thaha Rasool
Aug 26, 1998·Infection and Immunity·R W StichW C Brown

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