Cloning and expression of cDNA encoding the complete prepro-form of an isoform of Der f 1, the major group 1 allergen from house dust mite Dermatophagoides farinae

Bioscience, Biotechnology, and Biochemistry
T YasuharaY Okumura

Abstract

cDNA clones encoding a major house dust mite allergen, Der f 1, were isolated from a Dermatophagoides farinae cDNA library by plaque immunoscreening using rabbit anti-Der f 1 serum. The sequences cover the complete open reading frame encoding the prepro-form. The sequence is different from previously reported cDNA of Der f 1 in six bases and the encoded amino acid sequence is different in two residues. Pro-forms of Der f 1 and its mutant, in which the N-glycosylation motif was disrupted, expressed in Pichia pastoris were converted to the mature forms by an in vitro activation process and they showed significant IgE-binding. The biologically active rDer f 1 molecules would be useful for diagnostic testing and allergen-specific immunotherapy. In contrast, Der f 1 directly expressed in Escherichia coli without the prosequence had very low IgE binding. The hypoallergenic Der f 1 polypeptide could be useful for safer and more effective immunotherapy.

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Citations

Jan 28, 2006·International Archives of Allergy and Immunology·Toshiro TakaiHideoki Ogawa
May 14, 2011·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Yu-Chou DaiJiu-Yao Wang
Mar 20, 2012·Cell and Tissue Research·Deborah C HoltKatja Fischer
Jan 9, 2007·Plant Biotechnology Journal·David LienardVéronique Gomord
Mar 21, 2015·World Journal of Microbiology & Biotechnology·Chunfang LingAilin Tao
Dec 3, 2014·Protein Expression and Purification·Chunfang LingAilin Tao
Jul 20, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yuko KikuchiHideoki Ogawa
Nov 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Toshiro TakaiKo Okumura
Sep 19, 2017·Molecular Nutrition & Food Research·Yuan JinRichard E Goodman
Sep 28, 2020·Experimental Parasitology·DongLing NiuWanYu Zhang

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