Cloning and expression of draTG genes from Azospirillum lipoferum

Gene
H A FuR H Burris

Abstract

A genomic library of Azospirillum lipoferum was constructed with phage lambda EMBL4 as vector. From this library, the genes encoding dinitrogenase reductase ADP-ribosyltransferase (DRAT), draT, and dinitrogenase reductase-activating glycohydrolase (DRAG), draG, were cloned by hybridization with the heterologous probes of Rhodospirillum rubrum. As in R. rubrum, draT is located between draG and nifH, the gene encoding dinitrogenase reductase (a substrate for the DRAG/DRAT system). In the crude extract of Escherichia coli harboring the expression vector for this region, DRAT and DRAG enzyme activities were detected, confirming the identity of the cloned genes. Southern hybridization with genomic DNA from different Azospirillum spp., demonstrated a correlation between observable draTG hybridization and the biochemical demonstration of this covalent modification system.

References

Sep 1, 1978·The Biochemical Journal·P W LuddenR H Burris
Sep 1, 1989·Journal of Bacteriology·H A FuR H Burris
Aug 1, 1989·Molecular & General Genetics : MGG·W P FitzmauriceG P Roberts
Jan 1, 1989·Current Topics in Cellular Regulation·P W Ludden, G P Roberts
Mar 1, 1986·Journal of Bacteriology·A HartmannR H Burris
Jan 1, 1972·Methods in Enzymology·R H Burris
Jul 1, 1983·Analytical Biochemistry·A P Feinberg, B Vogelstein

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Citations

May 1, 1991·Molecular & General Genetics : MGG·A K TripathiW Klingmüller
Sep 1, 1994·Molecular and Cellular Biochemistry·P W Ludden
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H FuG P Roberts
May 18, 2011·BMC Microbiology·Fernando H Sant'annaIrene S Schrank
Jun 11, 2015·PloS One·Robert L RobsonToby H Richardson
Aug 1, 1994·Pacing and Clinical Electrophysiology : PACE·R J SungH Chun
Jul 15, 1997·FEMS Microbiology Letters·Y ZhangG P Roberts
Dec 1, 1995·Microbiological Reviews·M J Merrick, R A Edwards

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