PMID: 9524204Apr 3, 1998Paper

Cloning and expression of Escherichia coli 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase: identification of the pfs gene product

Biochimica Et Biophysica Acta
Kenneth A Cornell, Michael K Riscoe

Abstract

The enzyme 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase (EC 3.2.2.9) is responsible for cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S-adenosylhomocysteine (SAH). Based on amino acid sequence analysis of this enzyme from Klebsiella, we recently speculated that an open reading frame found in E. coli (designated pfs) encoded MTA/SAH nucleosidase. To explore this possibility, we amplified, cloned, and expressed the complete pfs gene from E. coli genomic DNA. The recombinant protein exhibited a molecular weight and Michaelis constants for MTA that are in agreement with those reported for native enzyme. From this biochemical evidence we confirm our original assignment of the pfs gene as encoding MTA/SAH nucleosidase.

References

Jul 1, 1991·Antimicrobial Agents and Chemotherapy·C J BacchiN Yarlett
Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S M Wurgler, C C Richardson
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 1, 1989·Annals of Human Genetics·D E Coulter-Karis, M S Hershfield
Jan 1, 1988·Advances in Experimental Medicine and Biology·V ZappiaM Cartenì-Farina
Nov 1, 1969·The Biochemical Journal·A E Pegg, H G Williams-Ashman
Apr 29, 1983·Biochemical and Biophysical Research Communications·D A CarsonN Kamatani
May 29, 1981·Biochemical and Biophysical Research Communications·A J FerroM R MacDonald
Feb 1, 1982·Biochemical Pharmacology·H G Williams-AshmanP Galletti
Jul 28, 1995·Science·R D FleischmannJ M Merrick
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·O I OlopadeS K Bohlander
Jul 1, 1994·Protein Science : a Publication of the Protein Society·A R Mushegian, E V Koonin
Nov 1, 1995·Antimicrobial Agents and Chemotherapy·J R SufrinC J Bacchi

❮ Previous
Next ❯

Citations

Jun 5, 2010·Applied Microbiology and Biotechnology·Takayuki AsaharaKiyoshi Matsuno
Mar 27, 2002·Journal of Bacteriology·Brooke A MurphyTina M Henkin
Jan 16, 2002·Journal of Bacteriology·Nathalie CadieuxRobert J Kadner
Apr 20, 2005·Journal of Bacteriology·Kelly BabbBrian Stevenson
Nov 20, 2009·The Journal of Biological Chemistry·Martin R ChallandPeter L Roach
Mar 2, 2010·Applied and Environmental Microbiology·Stefan KolEriko Takano
Mar 31, 2009·FEBS Letters·Martin R ChallandPeter L Roach
Dec 18, 2015·Epigenetics & Chromatin·Shai DuchinAmir Aharoni
Dec 21, 2010·Molecular Microbiology·Nikhat Parveen, Kenneth A Cornell
Oct 30, 2016·Metabolic Engineering·Narendranath BhokishamWilliam E Bentley
Oct 23, 2012·ACS Chemical Biology·Richard BaranTrent R Northen
Sep 13, 2016·Scientific Reports·A RuparellK R Hardie
Jun 6, 2018·Infection and Immunity·Jeffrey S BourgeoisDennis C Ko
Nov 9, 2018·Frontiers in Bioengineering and Biotechnology·M Kalim AkhtarGary J Loake
Jan 1, 2006·EcoSal Plus·Elise R Hondorp, Rowena G Matthews
Sep 1, 2008·EcoSal Plus·Kaj Frank JensenMartin WillemoËs
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·M K Berlyn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.