Cloning and expression of five heat shock protein genes in relation to cold hardening and development in the leafminer, Liriomyza sativa

Journal of Insect Physiology
Li-Hua HuangLe Kang

Abstract

The vegetable leafminer, Liriomyza sativae has spread worldwide, causing serious loss of agricultural productivity. Heat shock proteins (HSPs) play important roles in the environmental adaptation of various organisms, and to explore the functions of HSPs in relation to cold tolerance and development in L. sativae, three full-length cDNAs of small heat shock protein genes (ls-hsp19.5, ls-hsp20.8 and ls-hsp21.7) and two partial cDNAs of tcp1 (the hsp60 homolog, ls-tcp1alpha and ls-tcp1zeta) were cloned, and their transcriptional expression during cold hardening and development was examined by real time quantitative PCR. The open reading frames (ORFs) of ls-hsp19.5, ls-hsp20.8 and ls-hsp21.7 are 516, 543 and 573bp in length, encoding proteins with molecular weights (M.W.) of 19.5, 20.8 and 21.7kDa, respectively. The 956 and 323bp partial cDNAs were respectively sequenced from ls-tcp1alpha and ls-tcp1zeta. The expression profiles during cold hardening revealed that ls-tcp1s did not respond to cold stress. However, the three small hsps were significantly induced by cold, and ls-hsp20.8 was more cold-sensitive than the others. These results suggest that different shsp members may be responsible for cold stresses of different intensit...Continue Reading

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Citations

Dec 21, 2011·Environmental Entomology·Haihong WangRonald D Oetting
Mar 3, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Qi XuShigui Wang
Dec 10, 2015·Journal of Proteomics·Manisha MishraPradhyumna Kumar Singh
Mar 8, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kai LuQiang Zhou
Oct 24, 2014·Annual Review of Entomology·Allison M King, Thomas H MacRae
May 18, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kai LuQiang Zhou
Feb 5, 2019·PLoS Neglected Tropical Diseases·Meichun ZhangXiaoying Zheng

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