Cloning and expression of full length mouse utrophin: the differential association of utrophin and dystrophin with AChR clusters

FEBS Letters
W X GuoJ P Merlie

Abstract

We have cloned and sequenced mouse utrophin cDNA, and successfully expressed full length utrophin (400 kDa) in both muscle and non-muscle cells. The expression of recombinant utrophin is compared with that of its homologue, dystrophin (427 kDa). We demonstrate that recombinant utrophin is targeted into agrin-induced acetylcholine receptor (AChR) clusters, while recombinant dystrophin is evenly distributed along cell membranes in cultured Sol 8 muscle cells. This observation suggests that utrophin and dystrophin may interact with different cytoskeletal proteins. The C-terminal domains are found to be responsible for the association of utrophin with AChR clusters.

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Citations

Aug 21, 2001·Biochemical and Biophysical Research Communications·S P ChambersD R Love
Aug 2, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jatinderpal R DeolGeorge Karpati
Mar 11, 1999·Human Molecular Genetics·C N LumengJ S Chamberlain
Jan 29, 2014·The Journal of Cell Biology·Yoshimitsu KanaiNobutaka Hirokawa
Oct 21, 2016·International Journal of Molecular Sciences·Jacek DygutIrena Roterman
Sep 1, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Maria J MolnarGeorge Karpati
Mar 3, 2017·The Journal of Physical Chemistry. B·Yuan LiuSik Lok Lam
Jul 12, 2017·The Journal of Physical Chemistry Letters·Pei Guo, Sik Lok Lam
Aug 10, 2006·Journal of Muscle Research and Cell Motility·Bruno ConstantinChristian Cognard
Mar 7, 2003·American Journal of Physiology. Cell Physiology·Jutta G EbertUlrich Förstermann
Mar 28, 2002·Physiological Reviews·Derek J BlakeKay E Davies
Feb 19, 2000·Microscopy Research and Technique·F H AndradeH J Kaminski
Apr 14, 2005·The Journal of Biological Chemistry·Inna N Rybakova, James M Ervasti
Dec 10, 1999·The Journal of Biological Chemistry·K J AmannJ M Ervasti
Sep 8, 2001·American Journal of Physiology. Cell Physiology·A O GramoliniB J Jasmin

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