PMID: 9434124Jan 20, 1998Paper

Cloning and expression of human mitotic centromere-associated kinesin gene

Biochimica Et Biophysica Acta
I G KimY H Kim

Abstract

The human homologue of the hamster mitotic centromere-associated kinesin (HsMCAK) gene containing a central type motor domain was isolated from a Jurkat T-cell derived cDNA library. The HsMCAK gene has a predicted 723 amino acid open reading frame, encoding a 81 kDa protein that shares 79.2% homology with hamster MCAK. Unstimulated T lymphocytes contained no detectable HsMCAK-specific mRNA. Activation of resting T-cells by immobilized anti-CD3 resulted in the expression of a 2.9-kb transcript during the S phase of the cell cycle. The TPA-induced monocytic differentiation of U937 which also results in growth-arrest abruptly downregulates the expression of HsMCAK. Removal of TPA restored the growth of the cell through the retrodifferentiation process and the subsequent expression of HsMCAK. HsMCAK is expressed in tissues containing dividing cells, such as thymus, testis, small intestine, colon (mucosal lining), and placenta. These results suggest that the expression of HsMCAK is first detected in early S phase to support the proliferative response and is strictly regulated at the transcriptional level.

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Citations

Oct 18, 2002·Life Sciences·Li Jun ChengJia Hao Sha
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·H MikiN Hirokawa
Oct 1, 1992·Journal of Neuroendocrinology·W S Young
Jul 9, 2009·Cellular and Molecular Life Sciences : CMLS·Per HolmfeldtMartin Gullberg
Nov 19, 2009·Molecular Systems Biology·Martino BarencoMichael Hubank
Jul 11, 2006·The Journal of Biological Chemistry·Emily R HildebrandtM Andrew Hoyt
May 20, 2003·Traffic·Yulia Ovechkina, Linda Wordeman
Sep 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Pornchai O-charoenratBhuvanesh Singh

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